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NR0B1 Antibody (PA5-80865) in ICC/IF
Immunofluorescence staining of NR0B1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NR0B1 Polyclonal Antibody (Product # PA5-80865, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.
产品信息
PA5-80865
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to the center region of the Human NR0B1/DAX1
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A, Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2788131
产品详细信息
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human NR0B1/DAX1.
靶标信息
This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DAX 1; DSS-AHC critical region on the X chromosome protein 1; nuclear hormone receptor; Nuclear receptor DAX-1; Nuclear receptor subfamily 0 group B member 1; nuclear receptor subfamily 0, group B, member 1; R0B1
基因别名: AHC; AHCH; AHX; DAX-1; DAX1; DSS; GTD; HHG; NR0B1; NROB1; SRXY2
UniProt ID: (Human) P51843
Entrez Gene ID: (Human) 190
DNA binding
steroid hormone receptor activity
transcription corepressor activity
RNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
transcription factor binding
protein domain specific binding
DNA hairpin binding
steroid hormone receptor binding
protein homodimerization activity
sequence-specific DNA binding
C4 zinc finger nuclear receptor
DNA-binding transcription factor
zinc finger transcription factor
gene-specific transcriptional regulator
negative regulation of transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
steroid biosynthetic process
spermatogenesis
protein localization
gonad development
male gonad development
hypothalamus development
pituitary gland development
male sex determination
adrenal gland development
intracellular receptor signaling pathway
negative regulation of intracellular steroid hormone receptor signaling pathway
Leydig cell differentiation
response to immobilization stress
steroid hormone mediated signaling pathway
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of cell differentiation
negative regulation of transcription, DNA-templated
Sertoli cell differentiation
