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NR5A2 Antibody (PA5-49427) in WB
Western blot analysis of NR5A2 in various lysates. Samples were incubated with NR5A2 polyclonal antibody (Product # PA5-49427) using a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: mouse small intestine lysates; Lane 2: rat testis lysates. Predicted band size: 64 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Product Details
PA5-49427
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 200-233 amino acids from the Central region of Mouse Nr5a2.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2634881
Target Information
Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: fetoprotein transcription factor; FTZ F1; FTZ F1beta; FTZ-F1 beta; FTZ-F1 beta2 protein; hB1F 2; live; Liver receptor homolog 1; LRH-1; Nuclear receptor subfamily 5 group A member 2
Gene Aliases: AU020803; D1Ertd308e; Ftf; LRH-1; Lrh1; Nr5a2; UF2-H3B
UniProt ID: (Rat) Q9QWM1, (Mouse) P45448
Entrez Gene ID: (Rat) 60349, (Mouse) 26424
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
phospholipid binding
zinc ion binding
lipid binding
sequence-specific DNA binding
transcription regulatory region DNA binding
metal ion binding
C4 zinc finger nuclear receptor
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
bile acid metabolic process
intracellular receptor signaling pathway
epithelial cell differentiation
regulation of cell proliferation
cholesterol homeostasis
steroid hormone mediated signaling pathway
positive regulation of viral genome replication
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
pancreas morphogenesis
regulation of transcription from RNA polymerase II promoter
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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