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NSD1 Antibody (MA5-44597) in ICC/IF
Immunofluorescence analysis of NSD1 was performed using 70% confluent log phase NIH 3T3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NSD1 Recombinant Rabbit Monoclonal Antibody (1B1) (Product # MA5-44597) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Pl... View More
产品信息
MA5-44597
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
1B1
抗原
Recombinant protein within human NSD1 aa 1-200.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2931057
产品详细信息
Predicted band size: 297 kDa.
Positive Control: HEK293.
Subcellular Location: Nucleus.
靶标信息
This gene encodes a protein containing a SET domain, 2 LXXLL motifs, 3 nuclear translocation signals (NLSs), 4 plant homeodomain (PHD) finger regions, and a proline-rich region. The encoded protein enhances androgen receptor (AR) transactivation, and this enhancement can be increased further in the presence of other androgen receptor associated coregulators. This protein may act as a nucleus-localized, basic transcriptional factor and also as a bifunctional transcriptional regulator. Mutations of this gene have been associated with Sotos syndrome and Weaver syndrome. One version of childhood acute myeloid leukemia is the result of a cryptic translocation with the breakpoints occurring within nuclear receptor-binding Su-var, enhancer of zeste, and trithorax domain protein 1 on chromosome 5 and nucleoporin, 98-kd on chromosome 11. Two transcript variants encoding distinct isoforms have been identified for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Androgen receptor coactivator 267 kDa protein; androgen receptor-associated coregulator 267; Androgen receptor-associated protein of 267 kDa; H3-K36-HMTase; H4-K20-HMTase; Histone-lysine N-methyltransferase, H3 lysine-36 specific; Lysine N-methyltransferase 3B; NR-binding SET domain-containing protein; Nuclear receptor-binding SET domain-containing protein 1
基因别名: ARA267; KMT3B; NSD1; SOTOS; SOTOS1; STO
UniProt ID: (Human) Q96L73
Entrez Gene ID: (Human) 64324
RNA polymerase II core promoter sequence-specific DNA binding
chromatin binding
transcription cofactor activity
transcription corepressor activity
zinc ion binding
histone-lysine N-methyltransferase activity
estrogen receptor binding
histone methyltransferase activity (H4-K20 specific)
retinoic acid receptor binding
retinoid X receptor binding
thyroid hormone receptor binding
histone methyltransferase activity (H3-K36 specific)
androgen receptor binding
negative regulation of transcription from RNA polymerase II promoter
regulation of histone H3-K36 methylation
transcription, DNA-templated
histone H3-K36 methylation
histone methylation
regulation of peptidyl-serine phosphorylation
histone H4-K20 methylation
positive regulation of transcription, DNA-templated
regulation of RNA polymerase II regulatory region sequence-specific DNA binding
