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NSUN6 Antibody (PA5-96608) in WB
Western blot analysis of NSUN6 in extracts of various cell lines. Samples were incubated with NSUN6 Polyclonal antibody (Product # PA5-96608) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.
产品信息
PA5-96608
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 220-469 of human NSUN6 (NP_8723491)
偶联物
形式
Liquid
浓度
3.32 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2808410
产品详细信息
Immunogen sequence: NLPSALVSHV LNPQPGEKIL DLCAAPGGKT THIAALMHDQ GEVIALDKIF NKVEKIKQNA LLLGLNSIRA FCFDGTKAVK LDMVEDTEGE PPFLPESFDR ILLDAPCSGM GQRPNMACTW SVKEVASYQP LQRKLFTAAV QLLKPEGVLV YSTCTITLAE NEEQVAWALT KFPCLQLQPQ EPQIGGEGMR GAGLSCEQLK QLQRFDPSAV PLPDTDMDSL REARREDMLR LANKDSIGFF IAKFVKCKST; Positive Samples: Jurkat, HepG2, HeLa, 293T, Mouse testis
靶标信息
NSun6 is an RNA methyltransferase that catalyzes the methylation at position cytosine C72 in some tRNAs. NSUN6 has been reported to localize to the cytoplasm and largely colocalize with Golgi apparatus and pericentriolar matrix, indicating that NSUN6 modifies tRNAs in a late step in their biogenesis. NSun6 may also play a major role in bone metastasis and could be a valuable therapeutic target for bone metastasis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ARL5B antisense RNA 1; NOL1/NOP2/Sun and PUA domain-containing protein 1; NOL1/NOP2/Sun domain family 6; NOL1/NOP2/Sun domain family member 6; NOL1/NOP2/Sun domain family, member 6; NOP2/Sun domain family, member 6; nucleolar protein (NOL1/NOP2/sun) and PUA domains 1; putative methyltransferase NSUN6; tRNA (cytosine(72)-C(5))-methyltransferase NSUN6
基因别名: 4933403D21Rik; 4933414E04Rik; ARL5B-AS1; NOPD1; NSUN6
UniProt ID: (Human) Q8TEA1, (Mouse) Q7TS68
Entrez Gene ID: (Human) 221078, (Mouse) 74455
tRNA binding
RNA methyltransferase activity
S-adenosylmethionine-dependent methyltransferase activity
tRNA (cytosine-5-)-methyltransferase activity
rRNA (adenine-N6,N6-)-dimethyltransferase activity
molecular_function
RNA binding
protein C-terminal carboxyl O-methyltransferase activity
tRNA (guanine-N2-)-methyltransferase activity
methyltransferase activity
C-methyltransferase activity
N-methyltransferase activity
O-methyltransferase activity
S-methyltransferase activity
mRNA methyltransferase activity
tRNA methyltransferase activity
protein methyltransferase activity
2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity
rRNA methyltransferase activity
rRNA (uridine-2'-O-)-methyltransferase activity
rRNA (adenine-N6-)-methyltransferase activity
DNA-methyltransferase activity
tRNA (guanine-N1-)-methyltransferase activity
rRNA (cytosine-C5-)-methyltransferase activity
selenocysteine methyltransferase activity
arginine N-methyltransferase activity
protein-arginine N-methyltransferase activity
lysine N-methyltransferase activity
protein-lysine N-methyltransferase activity
tRNA (uracil) methyltransferase activity
tRNA (guanine) methyltransferase activity
tRNA (adenine) methyltransferase activity
tRNA (cytosine) methyltransferase activity
tRNA (adenine-N1-)-methyltransferase activity
rRNA (adenine) methyltransferase activity
rRNA (cytosine) methyltransferase activity
rRNA (guanine) methyltransferase activity
rRNA (uridine) methyltransferase activity
transferase activity
protein C-terminal leucine carboxyl O-methyltransferase activity
1-phenanthrol methyltransferase activity
protein-arginine N5-methyltransferase activity
methylarsonite methyltransferase activity
dimethylarsinite methyltransferase activity
4,5-dihydroxybenzo(a)pyrene methyltransferase activity
1-hydroxypyrene methyltransferase activity
1-hydroxy-6-methoxypyrene methyltransferase activity
demethylmenaquinone methyltransferase activity
cobalt-precorrin-6B C5-methyltransferase activity
cobalt-precorrin-7 C15-methyltransferase activity
cobalt-precorrin-5B C1-methyltransferase activity
cobalt-precorrin-3 C17-methyltransferase activity
dimethylamine methyltransferase activity
hydroxyneurosporene-O-methyltransferase activity
tRNA (adenine-57, 58-N(1)-) methyltransferase activity
methylamine-specific methylcobalamin:coenzyme M methyltransferase activity
trimethylamine methyltransferase activity
methanol-specific methylcobalamin:coenzyme M methyltransferase activity
monomethylamine methyltransferase activity
P-methyltransferase activity
Se-methyltransferase activity
2-phytyl-1,4-naphthoquinone methyltransferase activity
tRNA (uracil-2'-O-)-methyltransferase activity
tRNA (cytosine-2'-O-)-methyltransferase activity
phosphomethylethanolamine N-methyltransferase activity
tRNA (cytosine-3-)-methyltransferase activity
rRNA (cytosine-2'-O-)-methyltransferase activity
rRNA (cytosine-N4-)-methyltransferase activity
trihydroxyferuloyl spermidine O-methyltransferase activity
