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NT5C2 Antibody (PA5-112865) in ICC/IF
Immunocytochemichal analysis of NT5C2 in Hela cells using a Polyclonal antibody (Product # PA5-112865) at a dilution of 1:266. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.
产品信息
PA5-112865
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Human Cytosolic purine 5'-nucleotidase protein (487-553AA)
偶联物
形式
Liquid
浓度
3.77 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.03% ProClin 300
保存条件
-20°C or -80°C if preferred
运输条件
Wet ice
RRID
AB_2867599
靶标信息
Purine 5-prime-nucleotidase (EC 3.1.3.5) preferentially hydrolyzes inosine 5-prime-monophosphate (IMP) and other purine nucleotides, and is allosterically activated by various compounds, including ATP. The enzyme is exclusively located in the cytoplasmic matrix of cells and may have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 5'-nucleotidase (purine), cytosolic type B; cN-II; Cytosolic 5'-nucleotidase II; Cytosolic IMP/GMP-specific 5'-nucleotidase; Cytosolic nucleoside phosphotransferase 5'N; Cytosolic purine 5'-nucleotidase; High Km 5'-nucleotidase; IMP-specific 5'-NT; RP11-30H12.3
基因别名: 2010002I23Rik; cN-II; CnII; ENSMUSG00000025039; Gm9751; GMP; NT5B; NT5C2; NT5CP; PNT5; SPG45; SPG65
UniProt ID: (Human) P49902, (Mouse) Q3V1L4
Entrez Gene ID: (Human) 22978, (Mouse) 76952
