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NT5C2 Antibody (PA5-88185) in WB
Western blot analysis of NT5C2 in extracts of various cell lines. Samples were incubated with NT5C2 Polyclonal antibody (Product # PA5-88185) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
产品信息
PA5-88185
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 382-561 of human NT5C2 (NP_0363611)
偶联物
形式
Liquid
浓度
1.16 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2804725
产品详细信息
Immunogen sequence: WTDKSSLFEE LQSLDIFLAE LYKHLDSSSN ERPDISSIQR RIKKVTHDMD MCYGMMGSLF RSGSRQTLFA SQVMRYADLY AASFINLLYY PFSYLFRAAH VLMPHESTVE HTHVDINEME SPLATRNRTS VDFKDTDYKR HQLTRSISEI KPPNLFPLAP QEITHCHDED DDEEEEEEEE; Positive Samples: U-87MG, HT-29, HeLa, DU145, Mouse kidney, Rat testis; Cellular Location: Cytoplasm
靶标信息
Purine 5-prime-nucleotidase (EC 3.1.3.5) preferentially hydrolyzes inosine 5-prime-monophosphate (IMP) and other purine nucleotides, and is allosterically activated by various compounds, including ATP. The enzyme is exclusively located in the cytoplasmic matrix of cells and may have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 5'-nucleotidase (purine), cytosolic type B; 5'-nucleotidase, cytosolic II; cN-II; Cytosolic 5'-nucleotidase II; Cytosolic IMP/GMP-specific 5'-nucleotidase; Cytosolic nucleoside phosphotransferase 5'N; Cytosolic purine 5'-nucleotidase; High Km 5'-nucleotidase; IMP-specific 5'-NT; RP11-30H12.3
基因别名: 2010002I23Rik; cN-II; CnII; ENSMUSG00000025039; Gm9751; GMP; NT5B; NT5C2; NT5CP; PNT5; SPG45; SPG65
UniProt ID: (Human) P49902, (Mouse) Q3V1L4
Entrez Gene ID: (Human) 22978, (Mouse) 76952, (Rat) 100363253
