Invitrogen

NTF3 Polyclonal Antibody, PeproTech®

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NTF3 Antibody (500-P82-1MG) in ELISA

Sandwich ELISA: To detect Human NT-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of NTF3 Polyclonal Antibody (Product # 500-P82-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech NTF3 Polyclonal Antibody, Biotin (Product # 500-P82BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human NT-3.

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产品信息

500-P82-1MG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

中和实验 (Neu)

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产品规格

种属反应

Human

已发表种属

Mouse

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Human NT-3

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2930786

产品详细信息

AA Sequence of recombinant protein: MYAEHKSHRG EYSVCDSESL WVTDKSSAID IRGHQVTVLG EIKTGNSPVK QYFYETRCKE ARPVKNGCRG IDDKHWNSQC KTSQTYVRAL TSENNKLVGW RWIRIDTSCV CALSRKIGRT.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human NT-3. Anti-Human NT-3-specific antibody was purified by affinity chromatography employing an immobilized Human NT-3 matrix.

Sandwich ELISA: To detect Human NT-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human NT-3 (500-P82Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human NT-3.

Western Blot: To detect Human NT-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human NT-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.

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