Invitrogen

NUDT3 Polyclonal Antibody

NUDT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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NUDT3 Antibody (PA5-30435) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using NUDT3 (Product # PA5-30435) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Product Image Gallery

NUDT3 Antibody — Nudt3 down-regulation increases expression of mRNAs implicated in cell motility. (A) RNA levels of nine mRNAs implicated in cell migration tested in MCF-7 cells expressing nontargeting shRNA (ConKD, open bar), shRNA against Nudt3 (Nudt3KD, filled bar), or shRNA against Nudt4 (Nudt4KD, gray bar) are shown. (B) Western blot analysis of Nudt3 protein levels in ConKD or Nudt3KD MCF-7 cells ectopically expressing GFP, Flag-tagged wild-type Nudt3 (Nudt3), or mutant Nudt3 (Nudt3EE/QQ). The band for Flag-Nudt3EE/QQ runs slightly faster than that for Flag-Nudt3 likely due to differences in protein charge by the introduction of glutamic acid residues into the mutant protein. (C) A subset of the mRNAs implicated in cellular movement derived from the 144 mRNAs detected to increase greater than or equal to twofold by RNA-seq analysis in Nudt3KD are shown. (D) Transcript levels of the indicated mRNAs were determined by qRT-PCR in ConKD or Nudt3KD cells complemented with the indicated proteins. RNA levels in A and D are derived from at least three independent experiments and normalized to 18S rRNA and presented relative to the value in control cells set to one. Error bars represent ±SD. P-values are denoted by asterisks. (*) P < 0.05, (**) P < 0.01, (***) P < 0.001 (Student's t-test). {KD}

产品信息

PA5-30435

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 172 of Human NUDT3

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2547909

产品详细信息

Recommended positive controls: IMR32.

Predicted reactivity: Mouse (97%), Rat (96%), Dog (99%), Pig (99%), Chicken (95%), Rhesus Monkey (100%), Bovine (95%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

NUDT3 belongs to the MutT, or Nudix, protein family. Nudix proteins act as homeostatic checkpoints at important stages in nucleoside phosphate metabolic pathways, guarding against elevated levels of potentially dangerous intermediates, like 8-oxo-dGTP, which promotes AT-to-CG transversions (Safrany et al., 1998 ).

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Nucleolar Architecture Is Modulated by a Small Molecule, the Inositol Pyrophosphate 5-InsP₇.

Biomolecules

Sahu S,Gordon J,Gu C,Sobhany M,Fiedler D,Stanley RE,Shears SB

PA5-30435 was used in Immunohistochemistry (Paraffin), Western Blot to suggest that 5-InsP7 acts as an 'electrostatic glue' that binds together positively charged surfaces on separate proteins, overcoming mutual protein-protein electrostatic repulsion the latter phenomenon is a known requirement for the assembly of a non-membranous biomolecular condensate.

Thu Jan 12 00:00:00 UTC 2023

Nucleolar Architecture Is Modulated by a Small Molecule, the Inositol Pyrophosphate 5-InsP₇.

Biomolecules

Sahu S,Gordon J,Gu C,Sobhany M,Fiedler D,Stanley RE,Shears SB

Thu Jan 12 00:00:00 UTC 2023

Nudt3 is an mRNA decapping enzyme that modulates cell migration.

RNA (New York, N.Y.)

Grudzien-Nogalska E,Jiao X,Song MG,Hart RP,Kiledjian M

PA530435 was used in western blot to identify Nudt3 as a Nudix protein with mRNA decapping activity and examine its effects on breast cancer cell migration

Sun May 01 00:00:00 UTC 2016

生物信息学

蛋白别名: Ap6A hydrolase; diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase 1; Diadenosine hexaphosphate hydrolase; Diphosphoinositol polyphosphate phosphohydrolase 1; DIPP-1; Endopolyphosphatase; m7GpppN-mRNA hydrolase; m7GpppX diphosphatase; Nucleoside diphosphate-linked moiety X motif 3; nudix (nucleoside diphosphate linked moiety X)-type motif 3; Nudix motif 3

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基因别名: DIPP; DIPP-1; DIPP1; NUDT3

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UniProt ID: (Human) O95989

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Entrez Gene ID: (Human) 11165

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功能