Invitrogen

NUMB Monoclonal Antibody (S.925.4)

NUMB Antibody in Immunocytochemistry (ICC/IF)

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NUMB Antibody (MA5-14897) in ICC/IF

Immunofluorescence analysis of NUMB was performed using 70% confluent log phase DU 145 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with NUMB Monoclonal Antibody (S.925.4) (Product # MA5-14897) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Produ... View More

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NUMB Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. U87MG cells were transfected with NUMB siRNA and reduction of signal was observed in Western Blot using NUMB Monoclonal Antibody (Product # MA5-14897). {KD}

产品信息

MA5-14897

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

1:200

免疫沉淀 (IP)

1:50

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

S.925.4

抗原

Synthetic peptide corresponding to residues surrounding Phe482 of human Numb protein

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

88 µg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

<0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_10985378

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

Protein NUMB homolog is an evolutionarily conserved protein that controls the differentiation of neuronal progenitor cells. Recent studies have revealed that NUMB functions in clathrin-dependent endocytosis by binding to the AP-2 complex. NUMB is also expressed in postmitotic neurons and plays a role in axonal growth. Neural cells expressing Numb isoforms with short phosphotyrosine-binding (SPTB) domain undergo extensive neurite outgrowth, an effect that can be blocked by voltage-gated Ca2+ channel (VGCC) inhibitor or by Ca2+ chelator.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Mechanisms of integrin αVβ5 clustering in flat clathrin lattices.

Journal of cell science

Zuidema A,Wang W,Kreft M,Te Molder L,Hoekman L,Bleijerveld OB,Nahidiazar L,Janssen H,Sonnenberg A

MA5-14897 was used in Western Blotting to indicate that clustering of integrin αVβ5 in FCLs is dictated by the β5 subunit cytoplasmic domain, cellular tension and recruitment of specific adaptor proteins to the β5 subunit cytoplasmic domains.