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NUP50 Antibody (MA5-44782) in ICC/IF
Immunocytochemistry analysis of NUP50 in HeLa cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 &(8)451;, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Samples were incubated in NUP50 Monoclonal antibody (Product # MA5-44782) using a dilution of 1:50 in 2% negative goat serum overnight at 4 &(8)451; followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at a dilution of 1:1,000. Nuclear DNA was labelled in b... View More
产品信息
MA5-44782
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JE63-92
抗原
Synthetic peptide within human NUP50 aa 331-380/468.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2931239
靶标信息
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 50 kDa nucleoporin; CTA-268H5.7; DKFZP564A043; MGC39961; nuclear pore associated protein 60 kDa; Nuclear pore complex protein Nup50; Nuclear pore-associated protein 60 kDa-like; nuclear pore-associated protein 60L; nucleoporin 50 kDa; nucleoporin 50kD; nucleoporin 50kDa; Nucleoporin Nup50; nucleoprotein 50; Nucleoprotein 50kD; NUP50 protein; OTTHUMP00000199530
基因别名: 1700030K07Rik; AI413123; NPAP60; NPAP60L; NUP50; PRO1146; Rtp60
UniProt ID: (Human) Q9UKX7, (Rat) O08587, (Mouse) Q9JIH2
Entrez Gene ID: (Human) 10762, (Rat) 25497, (Mouse) 18141
mRNA export from nucleus
tRNA export from nucleus
protein import into nucleus
mitotic nuclear envelope disassembly
regulation of glucose transport
viral process
protein sumoylation
viral transcription
gene silencing by RNA
intracellular transport of virus
regulation of cellular response to heat
neural tube formation
protein transport
intracellular transport
mRNA transport
nucleocytoplasmic transport
