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NUP85 Antibody (PA5-88186) in WB
Western blot analysis of NUP85 in extracts of various cell lines. Samples were incubated with NUP85 Polyclonal antibody (Product # PA5-88186) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
产品信息
PA5-88186
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 427-656 of human NUP85 (NP_0791201)
偶联物
形式
Liquid
浓度
0.74 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2804726
产品详细信息
Immunogen sequence: CPELGRVSLE LHIERIPLNT EQKALKVLRI CEQRQMTEQV RSICKILAMK AVRNNRLGSA LSWSIRAKDA AFATLVSDRF LRDYCERGCF SDLDLIDNLG PAMMLSDRLT FLGKYREFHR MYGEKRFADA ASLLLSLMTS RIAPRSFWMT LLTDALPLLE QKQVIFSAEQ TYELMRCLED LTSRRPVHGE SDTEQLQDDD IETTKVEMLR LSLARNLARA IIREGSLEGS; Positive Samples: HT-29, U-87MG, A-549, LO2, HeLa, Mouse brain, Mouse testis, Rat testis; Cellular Location: Chromosome, Cytoplasm, Nucleus, Nucleus membrane, centromere, cytoskeleton, kinetochore, nuclear pore complex, spindle
靶标信息
Bidirectional transport of macromolecules between the cytoplasm and nucleus occurs through nuclear pore complexes (NPCs) embedded in the nuclear envelope. NPCs are composed of subcomplexes, and NUP85 is part of one such subcomplex, Nup107-160.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 85 kDa nucleoporin; FLJ12549; FROUNT; Nuclear pore complex protein Nup85; nucleoporin 85kDa; Nucleoporin Nup75; Nucleoporin Nup85; PCN; PCNT; pericentrin 1; Pericentrin-1
基因别名: FROUNT; NUP75; NUP85; PCNT1
UniProt ID: (Human) Q9BW27, (Mouse) Q8R480, (Rat) Q4QQS8
Entrez Gene ID: (Human) 79902, (Mouse) 445007, (Rat) 287830
mRNA export from nucleus
tRNA export from nucleus
protein import into nucleus
sister chromatid cohesion
mitotic nuclear envelope disassembly
regulation of glucose transport
viral process
protein sumoylation
viral transcription
lamellipodium assembly
gene silencing by RNA
positive regulation of transcription, DNA-templated
macrophage chemotaxis
intracellular transport of virus
regulation of cellular response to heat
transport
chemotaxis
protein transport
cytokine-mediated signaling pathway
mRNA transport
