Invitrogen

Naked1 Polyclonal Antibody

Naked1 Antibody in Immunocytochemistry (ICC/IF)

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Naked1 Antibody (PA5-113280) in ICC/IF

Immunocytochemichal analysis of Naked1 in HepG2 cells using a Polyclonal antibody (Product # PA5-113280) at a dilution of 1:200. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.

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产品信息

PA5-113280

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:5,000

免疫细胞化学 (ICC/IF)

1:50-1:200

酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Human Protein naked cuticle homolog 1 protein (269-384AA)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

3.52 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.03% ProClin 300

保存条件

-20°C or -80°C if preferred

运输条件

Wet ice

AB_2868014

靶标信息

In the mouse, Nkd is a Dishevelled (see DVL1; MIM 601365)-binding protein that functions as a negative regulator of the Wnt (see WNT1; MIM 164820)-beta-catenin-Tcf signaling pathway.

仅用于科研。不用于诊断过程。未经明确授权不得转售。