Invitrogen

Necdin Monoclonal Antibody (OTI5D1)

查看其他12个Necdin抗体

抗体检测数据 (18)

Necdin Antibody in Immunocytochemistry (ICC/IF)

Necdin Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 18

Necdin Antibody (MA5-26294) in ICC/IF

Immunofluorescent analysis of NDN in HeLa cells. Cells were probed with a NDN monoclonal antibody (Product # MA5-26294).

Necdin Antibody in Immunocytochemistry (ICC/IF)

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Necdin Antibody in Western Blot (WB)

Product Image Gallery

Necdin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of NDN in HeLa cells. Cells were probed with a NDN monoclonal antibody (Product # MA5-26294).

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human lymphoma tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a NDN monoclonal antibody (Product # MA5-26294).

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human endometrium tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a NDN monoclonal antibody (Product # MA5-26294).

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human breast tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a NDN monoclonal antibody (Product # MA5-26294).

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human ovary tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a NDN monoclonal antibody (Product # MA5-26294).

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded carcinoma of human prostate tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a NDN monoclonal antibody (Product # MA5-26294).

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded carcinoma of human bladder tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a NDN monoclonal antibody (Product # MA5-26294).

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded carcinoma of human thyroid tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a NDN monoclonal antibody (Product # MA5-26294).

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded carcinoma of human liver tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a NDN monoclonal antibody (Product # MA5-26294).

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded carcinoma of human lung tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a NDN monoclonal antibody (Product # MA5-26294).

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human endometrium tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a NDN monoclonal antibody (Product # MA5-26294).

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human lymph node tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a NDN monoclonal antibody (Product # MA5-26294).

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human prostate tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a NDN monoclonal antibody (Product # MA5-26294).

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human pancreas tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a NDN monoclonal antibody (Product # MA5-26294).

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human bladder tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a NDN monoclonal antibody (Product # MA5-26294).

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human kidney tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a NDN monoclonal antibody (Product # MA5-26294).

Necdin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human colon tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a NDN monoclonal antibody (Product # MA5-26294).

Necdin Antibody in Western Blot (WB) — Western blot analysis of NDN in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with NDN (Product # MA5-26294) monoclonal antibody.

产品信息

MA5-26294

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:4,000

-

免疫组化（石蜡） (IHC (P))

1:150

-

免疫细胞化学 (ICC/IF)

1:100

-

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

OTI5D1

抗原

Full length human recombinant protein of NDN produced in HEK293T cell

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 1% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2724818

靶标信息

This intronless gene is located in the Prader-Willi syndrome deletion region. It is an imprinted gene and is expressed exclusively from the paternal allele.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Necdin; necdin homolog; necdin, melanoma antigen (MAGE) family member; necdin-like protein; Prader-Willi syndrome chromosome region

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基因别名: HsT16328; NDN; PWCR

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UniProt ID: (Human) Q99608

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Entrez Gene ID: (Human) 4692

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RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding gamma-tubulin binding scaffold/adaptor protein

neuron migration respiratory system process transcription from RNA polymerase II promoter nervous system development axonal fasciculation central nervous system development negative regulation of cell proliferation glial cell migration post-embryonic development sensory perception of pain regulation of growth positive regulation of transcription from RNA polymerase II promoter neurotrophin TRK receptor signaling pathway axon extension multicellular organismal homeostasis genetic imprinting