Invitrogen

Nestin Monoclonal Antibody (3k1)

Nestin Antibody in Immunocytochemistry (ICC/IF)

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Nestin Antibody (MA1-26777) in ICC/IF

Immunofluorescence analysis of Nestin was performed using 70% confluent log phase Neural stem cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Nestin Monoclonal Antibody (3k1) (Product # MA1-26777) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product... View More

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产品信息

MA1-26777

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,500

免疫细胞化学 (ICC/IF)

5-10 µg/mL

流式细胞分析 (Flow)

2.5 µg per 10^6 cells

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

3k1

抗原

NS0 cells transfected with a fragment corresponding to residues 618-1618 of human Nestin.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 5% trehalose

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_795025

产品详细信息

Recommended positive controls: Human neural progenitors and A172 cells.

MA1-26777 will not react with rodent.

Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot- add 50% glycerol and store at -20ºC or below. Avoid freeze-thaw cycles.

靶标信息

Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.

仅用于科研。不用于诊断过程。未经明确授权不得转售。