Invitrogen

Nestin Monoclonal Antibody (SP103)

Nestin Antibody in Immunocytochemistry (ICC/IF)

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Nestin Antibody (MA5-16421) in ICC/IF

Immunofluorescence analysis of Nestin was performed using 70% confluent log phase Neural Stem cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Nestin Monoclonal Antibody (SP103) (Product # MA5-16421) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 48... View More

Nestin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

MA5-16421

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:500

免疫组化（石蜡） (IHC (P))

1:100

免疫细胞化学 (ICC/IF)

1:200

流式细胞分析 (Flow)

1:50

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

SP103

抗原

Synthetic peptide corresponding to C-terminus of human nestin.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

纯化类型

Protein A/G

保存液

PBS, pH 7.6, with 1% BSA

内含物

0.1% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2537940

产品详细信息

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is kidney tissue.

靶标信息

Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.

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