Invitrogen

NeuN Polyclonal Antibody

NeuN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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NeuN Antibody (PA5-78639) in IHC (P)

NeuN Polyclonal Antibody detects NeuN protein expression by immunohistochemical analysis. Sample: Paraffin-Embedded adult mouse retina. Green: NeuN protein stained by NeuN Polyclonal Antibody (Product # PA5-78639) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

NeuN Antibody in Immunohistochemistry (Frozen) (IHC (F))

NeuN Antibody in Immunocytochemistry (ICC/IF)

NeuN Antibody in Immunohistochemistry (IHC)

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NeuN Antibody — Antibody specificity was demonstrated by the detection of differential basal expression of the target across cell lines and tissue lysates tested owing to their inherent genetic constitution. Relative expression of RNA binding protein fox-1 homolog 3 was observed in PC-12 cell line and Mouse and Rat Cerebellum and Brain tissue lysates but not in C2C12 or Mouse and Rat Heart tissue lysates, using Anti-NeuN Polyclonal Antibody (Product # PA5-78639) in Western Blot. {RE}

产品信息

PA5-78639

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫组化（冰冻） (IHC (F))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Mouse NeuN. The exact sequence is proprietary.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.42 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2736207

产品详细信息

Positive Control: mouse brain, rat brain

Predicted Reactivity: Rhesus Monkey (100%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Fox-3, also known as NEUN, HRNBP3, RBFOX3 (RNA binding protein, fox-1) or Fox-1 homolog C, is a 350 amino acid protein that contains one RRM (RNA recognition motif) domain. Localized to both the nucleus and cytoplasm, Fox-3 is suggested to regulate alternative splicing events. Fox-3 is encoded by a gene located on human chromosome 17, which is comprised over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and LiFraumeni syndrome. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Activation of astrocytes in the basal forebrain in mice facilitates isoflurane-induced loss of consciousness and prolongs recovery.

BMC anesthesiology

Lin J,Cheng X,Wang H,Du L,Li X,Zhao G,Xie C

PA5-78639 was used in Immunohistochemistry to suggest that astrocytes in the BF region are involved in isoflurane anesthesia and may be a potential target for regulating the consciousness state of anesthesia.

Tue Jun 20 00:00:00 UTC 2023

Dl-3-n-butylphthalide attenuates brain injury caused by cortical infarction accompanied by cranial venous drainage disturbance.

Stroke and vascular neurology

Song K,Zeng X,Xie X,Zhu R,Liang J,Chen G,Huang L

PA5-78639 was used in Immunohistochemistry and Western Blotting to explore the potential mechanism of the adverse effects of cerebrovenous disorders in cerebral infarction and explore the protective effect of Dl-3-n-butylphthalide on cerebral infarction accompanied through cerebral venous disturbance.

Wed Jun 01 00:00:00 UTC 2022

Dl-3-n-butylphthalide attenuates brain injury caused by cortical infarction accompanied by cranial venous drainage disturbance.

Stroke and vascular neurology

Song K,Zeng X,Xie X,Zhu R,Liang J,Chen G,Huang L

Wed Jun 01 00:00:00 UTC 2022

CRISPR/dCas9-Dnmt3a-mediated targeted DNA methylation of APP rescues brain pathology in a mouse model of Alzheimer's disease.

Translational neurodegeneration

Park H,Shin J,Kim Y,Saito T,Saido TC,Kim J

Published figure using NeuN polyclonal antibody (Product # PA5-78639) in Immunocytochemistry

Thu Sep 15 00:00:00 UTC 2022

Iatrogenic Iron Promotes Neurodegeneration and Activates Self-Protection of Neural Cells against Exogenous Iron Attacks.

Function (Oxford, England)

Xia M,Liang S,Li S,Ji M,Chen B,Zhang M,Dong C,Chen B,Gong W,Wen G,Zhan X,Zhang D,Li X,Zhou Y,Guan D,Verkhratsky A,Li B

PA5-78639 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to find that injection of iron dextran selectively decreased the presence of divalent metal transporter 1 (DMT1) in neurons through increasing the expression of Ndfip1, which degrades DMT1 and does not exist in glial cells.

Thu Jan 05 00:00:00 UTC 2023

Iatrogenic Iron Promotes Neurodegeneration and Activates Self-Protection of Neural Cells against Exogenous Iron Attacks.

Function (Oxford, England)

Xia M,Liang S,Li S,Ji M,Chen B,Zhang M,Dong C,Chen B,Gong W,Wen G,Zhan X,Zhang D,Li X,Zhou Y,Guan D,Verkhratsky A,Li B

Thu Jan 05 00:00:00 UTC 2023

Effects of cochlear hair cell ablation on spatial learning/memory.

Scientific reports

Qian ZJ,Ricci AJ

PA5-78639 was used in Immunohistochemistry-immunofluorescence to describe the previously undocumented effect of isolated SNHL on cognitive processes that do not directly rely on auditory sensory input.

Thu Nov 26 00:00:00 UTC 2020

Effects of cochlear hair cell ablation on spatial learning/memory.

Scientific reports

Qian ZJ,Ricci AJ

Thu Nov 26 00:00:00 UTC 2020

生物信息学

蛋白别名: FLJ56884; FLJ58356; fox 1 homolog (C. elegans) 3; Fox-1 homolog C; Fox-3; fox1 homolog C; FOX3NeuN; hexaribonucleotide binding protein 3; hexaribonucleotide binding protein 3 (HRNBP3); Hexaribonucleotide-binding protein 3; NeuN antigen; neuronal nuclear antigen A60; neuronal nuclei; Neuronal nuclei antigen; RFOX3; RNA binding protein; RNA binding protein fox-1 homolog 3; RNA binding protein, fox-1 homolog (C. elegans) 3; RNA binding protein, fox-1 homolog 3

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基因别名: D11Bwg0517e; FOX-3; FOX3; HRNBP3; NEUN; Neuna60; RBFOX3; RGD1560070

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UniProt ID: (Human) A6NFN3, (Mouse) Q8BIF2

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Entrez Gene ID: (Human) 146713, (Rat) 287847, (Mouse) 52897

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功能