Rockland

Neuraminidase (Neu2) Polyclonal Antibody

Neuraminidase (Neu2) Antibody in Western Blot (WB)

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Neuraminidase (Neu2) Antibody (600-401-850) in WB

Western blot analysis using Rockland Immunochemicals Affinity Purified anti-Neu2 antibody to detect Neu-2 present in a lysate expressing human Neu2 (1.0 ul loaded). Molecular weight marker (not shown) indicates a band of the expected MW (43 kDa). The reactive lower molecular weight band is believed to represent a truncated form of this protein. The blot was incubated with a 1:500 dilution of the antibody ... View More

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产品信息

600-401-850

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500- 1:2,000

免疫组化 (IHC)

Assay-dependent

酶联免疫吸附实验 (ELISA)

1:10,000-1:50,000

免疫沉淀 (IP)

1:500- 1:2,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion near aa 100-125 of Human Neu2.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.9 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody reacts with human Neu2. Based on sequence we expect this antibody to react with neuraminidase from other sources, although specific reactivity has not been confirmed. Cross-reactivity against Neu1 has not yet been established. Neuraminidases are highly conserved in mammals and therefore cross reactivity is expected with mouse and rat Neu2.

靶标信息

This gene belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosolic localization was demonstrated by cell fractionation experiments.

仅用于科研。不用于诊断过程。未经明确授权不得转售。