Invitrogen

Neurocan Recombinant Rabbit Monoclonal Antibody (JE54-15)

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抗体检测数据 (3)

Neurocan Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Neurocan Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Neurocan Antibody (MA5-36178) in IHC (P)

Immunohistochemical analysis of Neurocan in paraffin-embedded human liver carcinoma tissue using a monoclonal antibody (Product #MA5-36178). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 3... View More

Neurocan Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Neurocan Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Neurocan Antibody in Western Blot (WB)

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Neurocan Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Neurocan in paraffin-embedded human liver carcinoma tissue using a monoclonal antibody (Product #MA5-36178). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Neurocan Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Neurocan in paraffin-embedded human brain tissue using a monoclonal antibody (Product #MA5-36178). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Neurocan Antibody in Western Blot (WB) — Western blot analysis of Neurocan using a Neurocan monoclonal antibody (Product #MA5-36178). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody at a dilution of 1:500 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

产品信息

MA5-36178

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

Assay-dependent

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JE54-15

抗原

Recombinant protein within Human Neurocan aa 1-96

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 0.05% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2890336

靶标信息

chondroitin sulfate proteoglycan that may play a role in cell adhesion and cell migration.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Chondroitin sulfate proteoglycan 3; chondroitin sulfate proteoglycan 3 (neurocan); Neurocan core protein; neurocan proteoglycan

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基因别名: CSPG3; NCAN; NEUR

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UniProt ID: (Human) O14594

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Entrez Gene ID: (Human) 1463

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extracellular matrix structural constituent calcium ion binding hyaluronic acid binding carbohydrate binding extracellular matrix glycoprotein

skeletal system development cell adhesion central nervous system development extracellular matrix organization glycosaminoglycan metabolic process chondroitin sulfate biosynthetic process chondroitin sulfate catabolic process dermatan sulfate biosynthetic process