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Neurotensin Receptor 2 Antibody (ANT-016-200UL) in ICC/IF
Expression of NTR2 in rat C6 glioma cells - Immunocytochemical staining of rat C6 glioma cells. A. Paraformaldehyde fixed and permeabilized rat C6 glioma cells were stained with Anti-Neurotensin Receptor 2 Antibody (#ANT-016), (1:200), followed by Alexa-555 conjugated goat- Anti-rabbit- secondary Antibody (orange staining). Nuclei were visualized with the cell permeable dye Hoechst 33342 (blue staining). B.... View More
Product Details
ANT-016-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
CGEQHSLVPLPQEAPE, corresponding to amino acid residues 377-392 of rat NTSR2, Intracellular, C-terminus
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
The protein encoded by this gene belongs to the G protein-coupled receptor family that activate a phosphatidylinositol-calcium second messenger system. Binding and pharmacological studies demonstrate that this receptor binds neurotensin as well as several other ligands already described for neurotensin NT1 receptor. However, unlike NT1 receptor, this gene recognizes, with high affinity, levocabastine, a histamine H1 receptor antagonist previously shown to compete with neurotensin for low-affinity binding sites in brain. These activities suggest that this receptor may be of physiological importance and that a natural agonist for the receptor may exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: High-affinity levocabastine-sensitive neurotensin receptor; low affinity neurotensin receptor; Low-affinity levocabastine-sensitive neurotensin receptor; Neurotensin receptor type 2; NT-R-2; NT2 receptor; NTR2; NTR2 receptor; NTRL
Gene Aliases: NT2R; Ntr2; NTRL; Ntsr2
UniProt ID: (Rat) Q63384, (Mouse) P70310
Entrez Gene ID: (Rat) 64636, (Mouse) 18217
phospholipase C-activating G-protein coupled receptor signaling pathway
neuropeptide signaling pathway
chemical synaptic transmission
regulation of membrane potential
positive regulation of MAPK cascade
positive regulation of ERK1 and ERK2 cascade
signal transduction
G-protein coupled receptor signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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