Invitrogen

Neutrophil elastase Polyclonal Antibody

抗体检测数据 (3)

Neutrophil elastase Antibody in Western Blot (WB)

Neutrophil elastase Antibody in Western Blot (WB)

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Neutrophil elastase Antibody (PA5-79198) in WB

Western blot analysis of Neutrophil elastase in mouse bone extract (lane 1) and mouse spleen extract (lane 2). Sample was incubated with Neutrophil elastase polyclonal antibody (Product # PA5-79198) at a dilution of 0.5 µg/mL. Signal development was performed using a chemiluminescence (ECL) kit.

Neutrophil elastase Antibody in Western Blot (WB)

Neutrophil elastase Antibody in Western Blot (WB)

Neutrophil elastase Antibody in Western Blot (WB)

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Neutrophil elastase Antibody in Western Blot (WB) — Western blot analysis of Neutrophil elastase in mouse bone extract (lane 1) and mouse spleen extract (lane 2). Sample was incubated with Neutrophil elastase polyclonal antibody (Product # PA5-79198) at a dilution of 0.5 µg/mL. Signal development was performed using a chemiluminescence (ECL) kit.

Neutrophil elastase Antibody in Western Blot (WB) — Western blot analysis of Neutrophil elastase in mouse spleen tissue. Sample was incubated with Neutrophil elastase polyclonal antibody (Product # PA5-79198) at a dilution of 1 µg/mL. Signal development was performed using Streptavidin-Biotin-Complex (SABC) kit and a Tanon 5200 system.

Neutrophil elastase Antibody in Western Blot (WB) — Western blot analysis of Neutrophil elastase in rat spleen tissue. Sample was incubated with Neutrophil elastase polyclonal antibody (Product # PA5-79198) at a dilution of 1 µg/mL. Signal development was performed using Streptavidin-Biotin-Complex (SABC) kit and a Tanon 5200 system.

产品信息

PA5-79198

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

-

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

-

产品规格

种属反应

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived mouse Elastase/ELANE/ELA2 recombinant protein (Position: S27-N265).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2746314

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

The neutrophil form of elastase is 218 amino acids long, with two asparagine-linked carbohydrate chains (see glycosylation). It is present in azurophil granules in the neutrophil cytoplasm. There appear to be two forms of neutrophil elastase, termed IIa and IIb.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: ELA2A; Elastase; elastase 2, neutrophil; Elastase-2; Elastase2; Leukocyte elastase; Neutrophil elastase; PE-1

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基因别名: Ela2; Elane; F430011M15Rik; NE

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UniProt ID: (Mouse) Q3UP87

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Entrez Gene ID: (Rat) 299606, (Mouse) 50701

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RNA polymerase II transcription corepressor activity protease binding molecular_function endopeptidase activity serine-type endopeptidase activity heparin binding peptidase activity cytokine binding serine-type peptidase activity hydrolase activity serine protease

参与通路

negative regulation of transcription from RNA polymerase II promoter response to yeast acute inflammatory response to antigenic stimulus proteolysis phagocytosis biological_process response to UV response to lipopolysaccharide defense response to bacterium negative regulation of growth of symbiont in host negative regulation of chemokine biosynthetic process negative regulation of interleukin-8 biosynthetic process positive regulation of interleukin-8 biosynthetic process positive regulation of smooth muscle cell proliferation positive regulation of immune response defense response to fungus leukocyte migration neutrophil mediated killing of fungus transcription from RNA polymerase II promoter