Invitrogen

Neutrophil elastase Polyclonal Antibody

Neutrophil elastase Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Neutrophil elastase Antibody (PA5-87158) in IHC (P)

Immunohistochemistry analysis of Neutrophil elastase in paraffin-embedded Human colon carcinoma. Samples were incubated with Neutrophil elastase polyclonal antibody (Product # PA5-87158) using a dilution of 1:25. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Neutrophil elastase Antibody in Western Blot (WB)

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产品信息

PA5-87158

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:50-1:200

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流式细胞分析 (Flow)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide of human Neutrophil Elastase (ELANE).

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 50% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2803908

产品详细信息

Purity is > 95% by SDS-PAGE.

靶标信息

The neutrophil form of elastase is 218 amino acids long, with two asparagine-linked carbohydrate chains (see glycosylation). It is present in azurophil granules in the neutrophil cytoplasm. There appear to be two forms of neutrophil elastase, termed IIa and IIb.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Sputum from People with Cystic Fibrosis Reduces the Killing of Methicillin-Resistant Staphylococcus aureus by Neutrophils and Diminishes Phagosomal Production of Reactive Oxygen Species.

Pathogens (Basel, Switzerland)

Fantone KM,Goldberg JB,Stecenko AA,Rada B

PA5-87158 was used in Flow Cytometry to identify the airway environment as an important factor that restricts neutrophils' oxidative microbicidal activities in CF against MRSA.

Sat Sep 09 00:00:00 UTC 2023

Dual oxidase 1 is dispensable during Mycobacterium tuberculosis infection in mice.

Frontiers in immunology

Gupta T,Sarr D,Fantone K,Ashtiwi NM,Sakamoto K,Quinn FD,Rada B

PA5-87158 was used in Immunohistochemistry to demonstrate that while Duox1 might influence leukocyte recruitment to inflammatory cell aggregates, Duox1 is dispensable for the overall clinical course of Mtb lung infection in a mouse model.

Mon Mar 27 00:00:00 UTC 2023

Suppression of neutrophils by sodium exacerbates oxidative stress and arthritis.

Frontiers in immunology

Zlatar L,Mahajan A,Muñoz-Becerra M,Weidner D,Bila G,Bilyy R,Titze J,Hoffmann MH,Schett G,Herrmann M,Steffen U,Muñoz LE,Knopf J

PA5-87158 was used in Immunocytochemistry-immunofluorescence to investigate the effect of sodium chloride (NaCl) on neutrophil viability and functions in vitro, and in vivo employing the murine K/BxN-serum transfer arthritis (STA) model.

Tue Aug 22 00:00:00 UTC 2023

IgA Complexes Induce Neutrophil Extracellular Trap Formation More Potently Than IgG Complexes.

Frontiers in immunology

Gimpel AK,Maccataio A,Unterweger H,Sokolova MV,Schett G,Steffen U

PA5-87158 was used in Immunocytochemistry to compare the capability of IgA and IgG complexes formed by heat aggregation to induce neutrophil extracellular trap(NET) formation.

Thu Feb 24 00:00:00 UTC 2022

生物信息学

蛋白别名: Bone marrow serine protease; ELA2A; Elastase; elastase 2, neutrophil; Elastase-2; Elastase2; granulocyte-derived elastase; HLE; Human leukocyte elastase; Leukocyte elastase; Medullasin; Neutrophil elastase; PE-1; PMN elastase; polymorphonuclear elastase

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基因别名: ELA2; ELANE; F430011M15Rik; GE; HLE; HNE; NE; PMN-E; SCN1

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UniProt ID: (Human) P08246, (Mouse) Q3UP87

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Entrez Gene ID: (Human) 1991, (Rat) 299606, (Mouse) 50701

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