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Ngp Antibody (600-401-GW9) in WB
Western Blot of Rabbit anti-Ngp antibody. Lane 1: MW ladder (opal pre-stained) p/n (MB-210-0500). Lane 2: 32D lysate. Load: 10 µg per lane. Primary antibody: Ngp antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody (p/n 611-103-122) at 1:70,000 for 45 min at RT. Block: BlockOut (p/n MB-070) overnight at 4°C. Predicted/Observed size: 17, 20 kDa for Ngp. Higher banding due to glycosylation. ~125 kDa non-specific.
产品信息
600-401-GW9
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Affinity purified Anti-Ngp antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein of mouse Ngp protein.
偶联物
形式
Liquid
浓度
1.1 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Ngp is directed against full-length mouse Ngp. A BLAST analysis was used to suggest reactivity with this protein in Ngp based on 100% homology match for the immunogen sequence.
靶标信息
A proteomic study in 2011 determined a direct correlation between NGP mRNA levels and myeloid-derived suppressor cells. Specifically cathepsin B, a catalytic mechanism and potentially biomarker in a variety of cancers and amyloid plaque development, was inhibited by NGP. The significance of NGP as a negative regulator of tumor metastasis makes this antibody ideal for investigators involved in cancer research, as well as Alzheimer's research.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: clone B6; Cystatin-like protein; Myeloid bactenecin protein; myeloid granule protein; Myeloid secondary granule protein; Neutrophilic granule protein; NGP
基因别名: bectenecin; Ngp
UniProt ID: (Mouse) O08692
Entrez Gene ID: (Mouse) 18054
