Invitrogen

Nicastrin Polyclonal Antibody

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Nicastrin Antibody (PA1-758) in WB

Western blot was performed using Anti-Nicastrin Polyclonal Antibody (Product # PA1-758) and a 120 kDa band corresponding to Nicastrin was observed across cell lines and tissues tested along with an uncharacterized band (*) at 55 kDa. Whole cell extracts (60 µg lysate) of HeLa (Lane 1), IMR-32 (Lane 2), NIH/3T3 (Lane 3), L6 (Lane 4), Mouse Brain (Lane 5) and Rat Brain (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Produ... View More

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产品信息

PA1-758

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

查看11篇已发表文章 11篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic Peptide: C(688) I N A K A D V L F I A P R E P G A V S Y(708)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2149570

产品详细信息

PA1-758 detects nicastrin from human, mouse and rat samples.

PA1-758 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~110 kDa protein representing nicastrin.

PA1-758 immunizing peptide corresponds to amino acid residues 688-708 from human nicastrin. There is a single amino acid substitution between the human and mouse sequence. PA1-758 immunizing peptide (Cat. # PEP-131) is available for use in neutralization and control experiments.

靶标信息

NCSTN (Nicastrin) is a gene that encodes a type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Mutations in this gene are associated with familial acne inversa. A pseudogene of this gene is present on chromosome 21.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Upregulation of APP endocytosis by neuronal aging drives amyloid-dependent synapse loss.

Journal of cell science

Burrinha T,Martinsson I,Gomes R,Terrasso AP,Gouras GK,Almeida CG

PA1-758 was used in Western Blot to identify APP endocytosis upregulation as a potential mechanism of neuronal aging and, thus, a novel target to prevent late-onset Alzheimer's disease.

Sat May 01 00:00:00 UTC 2021

Bin1 and CD2AP polarise the endocytic generation of beta-amyloid.

EMBO reports

Ubelmann F,Burrinha T,Salavessa L,Gomes R,Ferreira C,Moreno N,Guimas Almeida C

Published figure using Nicastrin polyclonal antibody (Product # PA1-758) in Western Blot

Sun Jan 01 00:00:00 UTC 2017

Presenilin transmembrane domain 8 conserved AXXXAXXXG motifs are required for the activity of the γ-secretase complex.

The Journal of biological chemistry

Marinangeli C,Tasiaux B,Opsomer R,Hage S,Sodero AO,Dewachter I,Octave JN,Smith SO,Constantinescu SN,Kienlen-Campard P

PA1-758 was used in western blot to investigate presenilin assembly and catalytic activity

Fri Mar 13 00:00:00 UTC 2015

Ubiquilin-1 modulates γ-secretase-mediated ε-site cleavage in neuronal cells.

Biochemistry

Viswanathan J,Haapasalo A,Kurkinen KM,Natunen T,Mäkinen P,Bertram L,Soininen H,Tanzi RE,Hiltunen M

PA1-758 was used in western blot to study the ability of a variant of ubiquilin-1 to increase gamma-secretase-mediated cleavage of APP at the epsilon site

Tue Jun 04 00:00:00 UTC 2013

Presenilins regulate neurotrypsin gene expression and neurotrypsin-dependent agrin cleavage via cyclic AMP response element-binding protein (CREB) modulation.

The Journal of biological chemistry

Almenar-Queralt A,Kim SN,Benner C,Herrera CM,Kang DE,Garcia-Bassets I,Goldstein LS

PA1-758 was used in western blot to study the role of CREB in the mechanism by which neurotrypsin expression and agarin proteolysis is modulated by presenilins

Fri Dec 06 00:00:00 UTC 2013

Presenilins regulate neurotrypsin gene expression and neurotrypsin-dependent agrin cleavage via cyclic AMP response element-binding protein (CREB) modulation.

The Journal of biological chemistry

Almenar-Queralt A,Kim SN,Benner C,Herrera CM,Kang DE,Garcia-Bassets I,Goldstein LS

PA1-758 was used in western blot to study the role of CREB in the mechanism by which neurotrypsin expression and agarin proteolysis is modulated by presenilins

Fri Dec 06 00:00:00 UTC 2013

Chronic administration of anti-stroke herbal medicine TongLuoJiuNao reduces amyloidogenic processing of amyloid precursor protein in a mouse model of Alzheimer's disease.

PloS one

He P,Li P,Hua Q,Liu Y,Staufenbiel M,Li R,Shen Y

PA1-758 was used in western blot to study the effects of the herbal medicine TongLuoJuiNao on levels of BACE1, gamma secretase components, and beta-amyloid production in a murine model of Alzheimer's disease

Tue Dec 03 00:00:00 UTC 2013

Potent amyloidogenicity and pathogenicity of Aβ43.

Nature neuroscience

Saito T,Suemoto T,Brouwers N,Sleegers K,Funamoto S,Mihira N,Matsuba Y,Yamada K,Nilsson P,Takano J,Nishimura M,Iwata N,Van Broeckhoven C,Ihara Y,Saido TC

PA1-758 was used in western blot to investigate the contribution of A beta 43 to Alzheimer disease pathology

Sun Jul 03 00:00:00 UTC 2011

Calsenilin regulates presenilin 1/γ-secretase-mediated N-cadherin ε-cleavage and β-catenin signaling.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Jang C,Choi JK,Na YJ,Jang B,Wasco W,Buxbaum JD,Kim YS,Choi EK

PA1-758 was used in western blot to identify the role of Calsenilin in N-cadherin cleavage in neuroblastomas

Thu Dec 01 00:00:00 UTC 2011

Ubiquilin 1 modulates amyloid precursor protein trafficking and Abeta secretion.

The Journal of biological chemistry

Hiltunen M,Lu A,Thomas AV,Romano DM,Kim M,Jones PB,Xie Z,Kounnas MZ,Wagner SL,Berezovska O,Hyman BT,Tesco G,Bertram L,Tanzi RE

PA1-758 was used in western blot to investigate the role of ubiquilin 1 during the amyloid precursor protein trafficking and Abeta secretion.

Fri Oct 27 00:00:00 UTC 2006

The presenilin proteins are components of multiple membrane-bound complexes that have different biological activities.

The Journal of biological chemistry

Gu Y,Sanjo N,Chen F,Hasegawa H,Petit A,Ruan X,Li W,Shier C,Kawarai T,Schmitt-Ulms G,Westaway D,St George-Hyslop P,Fraser PE

PA1-758 was used in western blot to study multiple membrane-bound presenilin complexes and their biological activities.

Fri Jul 23 00:00:00 UTC 2004

APH-1 interacts with mature and immature forms of presenilins and nicastrin and may play a role in maturation of presenilin.nicastrin complexes.

The Journal of biological chemistry

Gu Y,Chen F,Sanjo N,Kawarai T,Hasegawa H,Duthie M,Li W,Ruan X,Luthra A,Mount HT,Tandon A,Fraser PE,St George-Hyslop P

PA1-758 was used in western blot to investigate the role of APH-1 during the formation of presenilin and nicastrin complexes.

Fri Feb 28 00:00:00 UTC 2003

APH-1 interacts with mature and immature forms of presenilins and nicastrin and may play a role in maturation of presenilin.nicastrin complexes.

The Journal of biological chemistry

Gu Y,Chen F,Sanjo N,Kawarai T,Hasegawa H,Duthie M,Li W,Ruan X,Luthra A,Mount HT,Tandon A,Fraser PE,St George-Hyslop P

PA1-758 was used in western blot to investigate the role of APH-1 during the formation of presenilin and nicastrin complexes.

Fri Feb 28 00:00:00 UTC 2003

生物信息学

蛋白别名: anterior pharynx-defective 2; Nicastrin; OTTHUMP00000031840

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基因别名: 9430068N19Rik; AA727311; Aph2; ATAG1874; D1Dau13e; KIAA0253; mKIAA0253; NCSTN; Nct; UNQ1874/PRO4317

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UniProt ID: (Human) Q92542, (Mouse) P57716, (Rat) Q8CGU6

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Entrez Gene ID: (Human) 23385, (Mouse) 59287, (Rat) 289231

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功能

endopeptidase activity protein binding peptidase activity

参与通路

myeloid cell homeostasis proteolysis membrane protein ectodomain proteolysis Notch signaling pathway protein processing T cell proliferation positive regulation of catalytic activity beta-amyloid metabolic process epithelial cell proliferation Notch receptor processing membrane protein intracellular domain proteolysis amyloid precursor protein catabolic process positive regulation of apoptotic process ephrin receptor signaling pathway

Nicastrin Polyclonal Antibody

Nicastrin Antibody (PA1-758) in WB

产品信息

PA1-758

免疫印迹 (WB)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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