Invitrogen

Nicastrin Recombinant Rabbit Monoclonal Antibody (JG68-33)

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Nicastrin Antibody (MA5-34754) in WB

Western blot was performed using Nicastrin Recombinant Rabbit Monoclonal Antibody (JG68-33) (Product # MA5-34754) and a 110-130 kDa band corresponding to Nicastrin was observed across cell lines. Membrane enriched extracts (30 µg lysate) of THP-1 (Lane 1), HeLa (Lane 2), T98G (Lane 3), Hep G2 (Lane 4), NK-92 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB33001) by iBlot™ 3 Western Blot Transfer Device ... View More

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产品信息

MA5-34754

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JG68-33

抗原

Recomninant protein within Human Nicastrin aa 5-112

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles, store in dark

运输条件

Wet ice

AB_2848662

产品详细信息

Positive Control: THP-1.

靶标信息

NCSTN (Nicastrin) is a gene that encodes a type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Mutations in this gene are associated with familial acne inversa. A pseudogene of this gene is present on chromosome 21.

仅用于科研。不用于诊断过程。未经明确授权不得转售。