Invitrogen

Nidogen Polyclonal Antibody

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Nidogen Antibody (PA5-97016) in WB

Western blot analysis of Nidogen in extracts of various cell lines. Samples were incubated with Nidogen Polyclonal antibody (Product # PA5-97016) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

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产品信息

PA5-97016

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 110-400 of human NID1 (NP_0024992)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.66 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2808818

产品详细信息

Immunogen sequence: DLDTTDGLGK VYYREDLSPS ITQRAAECVH RGFPEISFQP SSAVVVTWES VAPYQGPSRD PDQKGKRNTF QAVLASSDSS SYAIFLYPED GLQFHTTFSK KENNQVPAVV AFSQGSVGFL WKSNGAYNIF ANDRESVENL AKSSNSGQQG VWVFEIGSPA TTNGVVPADV ILGTEDGAEY DDEDEDYDLA TTRLGLEDVG TTPFSYKALR RGGADTYSVP SVLSPRRAAT ERPLGPPTER TRSFQLAVET FHQQHPQVID VDEVEETGVV FSYNTDSRQT CANNRHQCSV H; Positive Samples: LO2, 293T; Cellular Location: basement membrane, extracellular matrix, extracellular space

靶标信息

This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix.

仅用于科研。不用于诊断过程。未经明确授权不得转售。