Invitrogen

NuMA Polyclonal Antibody

NuMA Antibody in Immunocytochemistry (ICC/IF)

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NuMA Antibody (PA5-22285) in ICC/IF

Immunofluorescence analysis of NuMA was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NuMA Polyclonal Antibody (Product # PA5-22285) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1... View More

NuMA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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NuMA Antibody — Antibody specificity was demonstrated by detection of differential localisations of the target across mitosis. Differential localisation of NuMA was tested in HeLa cells in different phases of cell cycle, and was observed to be nuclear in the interphase cells, localised to the spindle poles in metaphasic cells, and loss of this upon dissolution of the spindle apparatus with progression through Anaphase, using NuMA Polyclonal Antibody (Product # PA5-22285) in immunofluorescence. {RE}

产品信息

PA5-22285

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:10,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 339 of NuMA (Uniprot ID#Q14980)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.54 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11157241

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2, HeLa, HeLa (100 ng/ml Nocodazole treatment for 24 hr).

Predicted reactivity: Mouse (81%), Rat (83%), Bovine (89%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

NuMA (nuclear mitotic apparatus) is a long coil-coiled protein that plays a role in the interphase and mitosis phases of a cell cycle. In interphase, it has been hypothesized to play a structural role in the nucleoskeleton that may be important in nuclear organization and functions. During mitosis, it is associated with spindle microtubule organization and chromosome positioning.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Identification of growth hormone receptor as a relevant target for precision medicine in low-EGFR expressing glioblastoma.

Clinical and translational medicine

Verreault M,Segoviano Vilchis I,Rosenberg S,Lemaire N,Schmitt C,Guehennec J,Royer-Perron L,Thomas JL,Lam TT,Dingli F,Loew D,Ducray F,Paris S,Carpentier C,Marie Y,Laigle-Donadey F,Rousseau A,Pigat N,Boutillon F,Bielle F,Mokhtari K,Frank SJ,de Reyniès A,Hoang-Xuan K,Sanson M,Goffin V,Idbaih A

PA5-22285 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to identify that growth hormone receptor (GHR) signalling defines a distinct molecular subset of GBMs devoid of EGFR overexpression.

Fri Jul 01 00:00:00 UTC 2022

A genome-wide enrichment screen identifies NUMA1-loss as a resistance mechanism against mitotic cell-death induced by BMI1 inhibition.

PloS one

Gisler S,Maia ARR,Chandrasekaran G,Kopparam J,van Lohuizen M

PA5-22285 was used in Western Blotting to perform a drug resistance screen on mutagenized human haploid HAP1 cells treated with BMI1 inhibitor PTC-318 to find new genetic and mechanistic features associated with BMI1-dependent cancer cell proliferation.

Mon Jul 06 00:00:00 UTC 2020

A genome-wide enrichment screen identifies NUMA1-loss as a resistance mechanism against mitotic cell-death induced by BMI1 inhibition.

PloS one

Gisler S,Maia ARR,Chandrasekaran G,Kopparam J,van Lohuizen M

Mon Jul 06 00:00:00 UTC 2020

Generation of lung organoids from human pluripotent stem cells in vitro.

Nature protocols

Miller AJ,Dye BR,Ferrer-Torres D,Hill DR,Overeem AW,Shea LD,Spence JR

PA5-22285 was used in Immunohistochemistry to differentiate human pluripotent stem cells (hPSCs) into ventral-anterior foregut spheroids and further into two distinct types of organoids: human lung organoids and bud tip progenitor organoids.

Fri Feb 01 00:00:00 UTC 2019

生物信息学

蛋白别名: centrophilin stabilizes mitotic spindle in mitotic cells; NMP-22; Nuclear matrix protein-22; Nuclear mitotic apparatus protein; Nuclear mitotic apparatus protein 1; NuMA protein; SP-H antigen; structural nuclear protein

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基因别名: NMP-22; NMP22; NUMA; NUMA1

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UniProt ID: (Human) Q14980

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Entrez Gene ID: (Human) 4926

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功能

structural molecule activity protein binding microtubule binding cytoskeletal protein microtubule or microtubule-binding cytoskeletal protein

参与通路

establishment of mitotic spindle orientation nucleus organization mitotic nuclear division cell division meiotic cell cycle lung epithelial cell differentiation