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Nucleolin Antibody (MA1-20800) in ICC/IF
Immunofluorescence analysis of Nucleolin was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Nucleolin Monoclonal Antibody (4E2) (Product # MA1-20800) at 5 microgram/mL concentration in 0.1% BSA, incubated at 4 degree celsius over... View More
产品信息
MA1-20800
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Human
已发表种属
Bovine,
Human,
Rabbit
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
4E2
抗原
Human nucleolin protein from Raji cell extract.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS with 50% glycerol
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_560407
产品详细信息
Recommended positive controls: Raji cell extract.
This antibody does not react with mouse protein.
靶标信息
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: FLJ45706; FLJ59041; nucl antibody; Nucleolin; Protein C23
基因别名: BOS_2214; C23; NCL
UniProt ID: (Human) P19338
Entrez Gene ID: (Bovine) 497013, (Human) 4691
nucleotide binding
single-stranded DNA binding
RNA binding
calcium ion binding
protein binding
protein C-terminus binding
selenocysteine insertion sequence binding
rRNA primary transcript binding
telomeric DNA binding
histone binding
identical protein binding
laminin binding
poly(A) RNA binding
ErbB-4 class receptor binding
angiogenesis
endocytosis
spermatogenesis
positive regulation of tumor necrosis factor production
negative regulation of apoptotic process
positive regulation of transcription from RNA polymerase II promoter
cellular response to lipopolysaccharide
liver regeneration
positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter
regulation of rRNA processing
positive regulation of interleukin-6 secretion
