NeoBiotechnologies

Nucleolin Monoclonal Antibody (NCL, 902)

Nucleolin Antibody in Immunocytochemistry (ICC/IF)

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Nucleolin Antibody (4691-MSM1-P1) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis of HeLa cells using Nucleolin Monoclonal Antibody (Product # 4691-MSM1-P1). Green: AF488-labeled Ab. Blue: DAPI..

Nucleolin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Nucleolin Antibody in Flow Cytometry (Flow)

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产品信息

4691-MSM1-P1

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

免疫组化（石蜡） (IHC (P))

1-2 µg/mL

免疫细胞化学 (ICC/IF)

1-2 µg/mL

流式细胞分析 (Flow)

1-2 µg/10^6 cells

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

NCL, 902

抗原

Recombinant full-length human NCL protein

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

200 µg/mL

纯化类型

Protein A/G

保存液

PBS, pH 7.4, with 0.05% BSA

内含物

0.05% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

This antibody does not react with Mouse, Rat or Cow.

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

靶标信息

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

仅用于科研。不用于诊断过程。未经明确授权不得转售。