Bethyl Laboratories

Nucleolin/NCL Polyclonal Antibody

Nucleolin/NCL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Nucleolin/NCL Antibody (A300-710A-T) in IHC (P)

Detection of human Nucleolin by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-Nucleolin (Product # A300-710A) (Lot 1) used at a dilution of 1:200 (1 µg/mL). Detection: DAB.

Nucleolin/NCL Antibody in Western Blot (WB)

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产品信息

A300-710A-T

应用

建议稀释比

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免疫印迹 (WB)

1:2,000-1:10,000

免疫组化 (IHC)

1:100-1:500

免疫组化（石蜡） (IHC (P))

1:100-1:500

免疫沉淀 (IP)

2-5 µg/mg lysate

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Region between residue 525 and 575 of human Nucleolin.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.20 mg/mL

纯化类型

Antigen affinity chromatography

保存液

TBS, pH 7.0 to 8.0, with 0.1% BSA

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Wet ice

产品详细信息

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Based on 100% sequence identity, this antibody is predicted to react with Rat, Golden hamster and Orangutan

靶标信息

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

仅用于科研。不用于诊断过程。未经明确授权不得转售。