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O-linked N-acetylglucosamine (O-GlcNAc) Antibody (MA1-038) in WB
Western blot analysis of O-GlcNAc was performed by loading 10 µg of HEK293T or C2C12 whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing O-GlcNAc (Product # MA1-038) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Memb... View More
产品信息
MA1-038
产品规格
种属反应
Chemical
已发表种属
Dog,
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
18B10.C7
抗原
SANM-conjugated linear peptide corresponding to human O-GlcNAc.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2536725
产品详细信息
MA1-038 has been successfully used in Western blot and immunoprecipitation applications with human samples. In Western blot, MA1-038 detects proteins with O-GlcNAc post-translational modification, and therefore will detect multiple specific bands.
靶标信息
O-linked N-acetylglucosamine (O-GlcNAc) is a posttranslational modification characterized by the attachment of N-acetylglucosamine to specific serine or threonine residues. Unlike other protein glycosylations, O-GlcNAc modifications occur within the nucleus and cytoplasm. They are found on many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins. O-GlcNAc glycosylations are thought to obscure phosphorylation sites, counteracting phosphorylation-dependent signaling pathways and protein interactions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: GlcNAc
