Invitrogen

OCT4 Monoclonal Antibody (T.631.9)

OCT4 Antibody in Immunocytochemistry (ICC/IF)

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OCT4 Antibody (MA5-14845) in ICC/IF

Immunofluorescent analysis of Oct-4A in mouse embryonic stem cells growing on mouse embryonic fibroblast feeder cells, using an oct-4A monoclonal antibody (Product # MA5-14845) (green). Actin filaments are labeled with a fluorescent red phalloidin.

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产品信息

MA5-14845

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:400

查看3篇已发表文章 3篇已发表文章

流式细胞分析 (Flow)

1:200

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产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

T.631.9

抗原

Recombinant protein specific to the amino terminus of human Oct-4A

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

58 µg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

<0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_10979606

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Dominantly acting KIF5B variants with pleiotropic cellular consequences cause variable clinical phenotypes.

Human molecular genetics

Flex E,Albadri S,Radio FC,Cecchetti S,Lauri A,Priolo M,Kissopoulos M,Carpentieri G,Fasano G,Venditti M,Magliocca V,Bellacchio E,Welch CL,Colombo PC,Kochav SM,Chang R,Barrick R,Trivisano M,Micalizzi A,Borghi R,Messina E,Mancini C,Pizzi S,De Santis F,Rosello M,Specchio N,Compagnucci C,McWalter K,Chung WK,Del Bene F,Tartaglia M

MA5-14845 was used in Immunocytochemistry to report three dominantly acting KIF5B variants (p.Asn255del, p.Leu498Pro and p.Leu537Pro) resulting in a clinically wide phenotypic spectrum, ranging from dilated cardiomyopathy with adult-onset ophthalmoplegia and progressive skeletal myopathy to a neurodevelopmental condition characterized by severe hypotonia with or without seizures.

Fri Jan 13 00:00:00 UTC 2023

Altered cytoskeletal arrangement in induced pluripotent stem cells (iPSCs) and motor neurons from patients with riboflavin transporter deficiency.

Disease models & mechanisms

Niceforo A,Marioli C,Colasuonno F,Petrini S,Massey K,Tartaglia M,Bertini E,Moreno S,Compagnucci C

Published figure using OCT4 monoclonal antibody (Product # MA5-14845) in Immunocytochemistry

Tue Jan 19 00:00:00 UTC 2021

Altered cytoskeletal arrangement in induced pluripotent stem cells (iPSCs) and motor neurons from patients with riboflavin transporter deficiency.

Disease models & mechanisms

Niceforo A,Marioli C,Colasuonno F,Petrini S,Massey K,Tartaglia M,Bertini E,Moreno S,Compagnucci C

Published figure using OCT4 monoclonal antibody (Product # MA5-14845) in Immunocytochemistry

Tue Jan 19 00:00:00 UTC 2021

Inhibition of SARS-CoV-2 infection in human iPSC-derived cardiomyocytes by targeting the Sigma-1 receptor disrupts cytoarchitecture and beating.

PeerJ

Salerno JA,Torquato T,Temerozo JR,Goto-Silva L,Karmirian K,Mendes MA,Sacramento CQ,Fintelman-Rodrigues N,Souza LRQ,Ornelas IM,Veríssimo CP,Aragão LGHS,Vitória G,Pedrosa CSG,da Silva Gomes Dias S,Cardoso Soares V,Puig-Pijuan T,Salazar V,Dariolli R,Biagi D,Furtado DR,Barreto Chiarini L,Borges HL,Bozza PT,Zaluar P Guimarães M,Souza TML,Rehen SK

MA5-14845 was used in Flow Cytometry to investigated the consequences of the inhibition of the Sigma-1 receptor, a ubiquitously expressed chaperone that plays a central role in cardiomyocyte function, in iPSC-derived human cardiomyocytes on the basis that it may affect SARS-CoV-2 replication.

Wed Nov 08 00:00:00 UTC 2023

Therapeutic manipulation of IKBKAP mis-splicing with a small molecule to cure familial dysautonomia.

Nature communications

Ajiro M,Awaya T,Kim YJ,Iida K,Denawa M,Tanaka N,Kurosawa R,Matsushima S,Shibata S,Sakamoto T,Studer L,Krainer AR,Hagiwara M

MA5-14845 was used in Immunocytochemistry to indicate that small synthetic molecules affecting phosphorylation state of SRSFs is available as a new therapeutic modality for mechanism-oriented precision medicine of splicing diseases.

Fri Jul 23 00:00:00 UTC 2021

Head and Neck Cancer Stem Cell-Enriched Spheroid Model for Anticancer Compound Screening.

Cells

Goričan L,Gole B,Potočnik U

MA5-14845 was used in Western Blotting to develop a cancer stem cell (CSC)-enriched spheroid model with a simple protocol, a microplate format compatible with multimodal detection systems, and a high detection signal, making it suitable for anti-CSC compounds' high-throughput screening (HTS).

Thu Jul 16 00:00:00 UTC 2020

Head and Neck Cancer Stem Cell-Enriched Spheroid Model for Anticancer Compound Screening.

Cells

Goričan L,Gole B,Potočnik U

Thu Jul 16 00:00:00 UTC 2020

Human Cardiac Organoids for Modeling Genetic Cardiomyopathy.

Cells

Filippo Buono M,von Boehmer L,Strang J,Hoerstrup SP,Emmert MY,Nugraha B

MA5-14845 was used in Flow cytometry/Cell sorting to demonstrate the generation of cardiac organoids using a triculture approach of human induced pluripotent stem-cell-derived cardiomyocytes (hiPS-CMs)- from healthy subjects and cardiomyopathy patients-human cardiac microvascular endothelial cells (HCMECs) and human cardiac fibroblasts (HCFs).

Mon Jul 20 00:00:00 UTC 2020

Human Cardiac Organoids for Modeling Genetic Cardiomyopathy.

Cells

Filippo Buono M,von Boehmer L,Strang J,Hoerstrup SP,Emmert MY,Nugraha B

Mon Jul 20 00:00:00 UTC 2020

生物信息学

蛋白别名: 3-Oct; 4-Oct; DADB-104B20.2; gp-9; HGNC:9221; MGC22487; NF-A3; Oct-3; Oct-4; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; OTF-3; OTTHUMP00000221150; OTTHUMP00000221151; POU class 5 homeobox 1 transcript variant OCT4B1; POU class 5 homeobox 1 transcript variant OCT4B2; POU class 5 homeobox 1 transcript variant OCT4B3; POU class 5 homeobox 1 transcript variant OCT4B4; POU class 5 homeobox 1 transcript variant OCT4B5; POU class 5 homeobox 1 transcript variant OCT4B6; POU domain class 5 transcription factor 1; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; pou-2; POU-type homeodomain-containing DNA-binding protein; pou2

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基因别名: NF-A3; Oct-3; Oct-3/4; Oct-4; OCT3; Oct3/4; OCT4; OTF-3; Otf-4; OTF3; Otf3-rs7; Otf3g; OTF4; POU5F1

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UniProt ID: (Human) Q01860, (Mouse) P20263

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Entrez Gene ID: (Human) 5460, (Mouse) 18999

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功能