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OPN-R Antibody (702184) in ICC/IF
Immunofluorescence analysis was performed on fixed and permeabilized U-2 OS cells for detection of endogenous osteopontin (cleaved) using Anti-osteopontin (cleaved) Recombinant Rabbit Monoclonal Antibody (Product # 702184, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of cleaved osteopontin protein (green), Panel b) is stained for nuclei (blue) using Slow... View More
产品信息
702184
产品规格
种属反应
Human,
Mouse
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
24H5L3
抗原
Peptide corresponding to Human SPP1 (aa 158-165)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2633064
产品详细信息
This antibody is predicted to react with Monkey, Rabbit and Bat.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
Osteopontin is a 34kDa extracellular matrix protein with a cell binding domain. It was originally identified as a major component of the non-collagenous organic bone matrix, however, it has subsequently been demonstrated in a wide range of normal adult tissue and body fluids. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases close to the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and provide a novel substrate for plasmin and cathepsin D.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 2AR; 44 kDa bone phosphoprotein; Bone sialoprotein 1; Calcium oxalate crystal growth inhibitor protein; early T-lymphocyte activation 1; Early T-lymphocyte activation 1 protein; Minopontin; Nephropontin; Osteopontin; osteopontin-like protein; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; Secreted phosphoprotein 1; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); secreted phosphoprotein 1 variant 6; SPP-1; SPP1/CALPHA1 fusion; Thrombin OPN-R; Urinary stone protein; Uropontin
基因别名: 2AR; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1; OP; OPN; Opnl; PSEC0156; Ric; Spp-1; SPP1
UniProt ID: (Human) P10451, (Mouse) P10923
Entrez Gene ID: (Human) 6696, (Mouse) 20750
osteoblast differentiation
inflammatory response
cell adhesion
embryo implantation
extracellular matrix disassembly
extracellular matrix organization
biomineral tissue development
response to vitamin D
positive regulation of bone resorption
decidualization
response to steroid hormone
negative regulation of collateral sprouting of intact axon in response to injury
ossification
response to organic substance
positive regulation of cell-substrate adhesion
neutrophil chemotaxis
negative regulation of apoptotic process
cellular response to fluid shear stress
