Invitrogen

OR5T1 Polyclonal Antibody

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抗体检测数据 (4)

OR5T1 Antibody in Immunocytochemistry (ICC/IF)

OR5T1 Antibody in Immunocytochemistry (ICC/IF)

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OR5T1 Antibody (PA5-99922) in ICC/IF

Immunocytochemistry analysis of OR5T1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with OR5T1 polyclonal antibody (Product # PA5-99922) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

OR5T1 Antibody in Immunocytochemistry (ICC/IF)

OR5T1 Antibody in Immunocytochemistry (ICC/IF)

OR5T1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

OR5T1 Antibody in Western Blot (WB)

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OR5T1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of OR5T1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with OR5T1 polyclonal antibody (Product # PA5-99922) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

OR5T1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of OR5T1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with OR5T1 polyclonal antibody (Product # PA5-99922) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

OR5T1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of OR5T1 in rat kidney tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with OR5T1 polyclonal antibody (Product # PA5-99922) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

OR5T1 Antibody in Western Blot (WB) — Western blot analysis of OR5T1 in mouse brain tissue lysate (left lane: treated with blocking peptide). Samples were incubated with OR5T1 polyclonal antibody (Product # PA5-99922).

产品信息

PA5-99922

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human OR5T1(Accession Q8NG75), corresponding to amino acid residues G76-L126.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2818855

产品详细信息

Antibody detects endogenous levels of total OR5T1.

靶标信息

Odorant receptor.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Olfactory receptor 5T1; Olfactory receptor OR11-179; olfactory receptor, family 5, subfamily T, member 1 pseudogene

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基因别名: OR11-179; OR5T1; OR5T1P

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UniProt ID: (Human) Q8NG75

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Entrez Gene ID: (Human) 390155

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adrenergic receptor activity olfactory receptor activity G-protein coupled receptor

cell-cell signaling detection of chemical stimulus involved in sensory perception of smell adenylate cyclase-activating adrenergic receptor signaling pathway