Invitrogen

ORC2 Monoclonal Antibody (3G6)

ORC2 Antibody in Immunocytochemistry (ICC/IF)

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ORC2 Antibody (MA5-17023) in ICC/IF

Immunofluorescence analysis of ORC2 was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ORC2 Monoclonal Antibody (Product # MA5-17023) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A-11006) at a dilution of 1:2000 for 45 minutes at room ... View More

ORC2 Antibody in Immunoprecipitation (IP)

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ORC2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with ORC2 siRNA and a decrease in signal intensity was observed in western blot application using Anti-ORC2 Monoclonal Antibody (Product # MA5-17023). {KD}

产品信息

MA5-17023

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫细胞化学 (ICC/IF)

1:200

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human

宿主/亚型

Rat / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

3G6

抗原

Full length protein (Human): ORC2 (hsORC)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2538495

产品详细信息

MA5-17023 reacts with ORC2 in human samples.

MA5-17023 has been successfully used in immunoprecipitation and Western blot applications.

The MA5-17023 immunogen is ORC2 (hsORC) produced in P3-x63Ag.8.653 cells.

靶标信息

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.

仅用于科研。不用于诊断过程。未经明确授权不得转售。