Invitrogen

OTC Polyclonal Antibody

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3 篇参考文献

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抗体检测数据 (3)
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OTC Antibody in Immunohistochemistry (Paraffin) (IHC (P))

OTC Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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OTC Antibody (PA5-28197) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using OTC (Product # PA5-28197) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

OTC Antibody in Immunohistochemistry (Paraffin) (IHC (P))

OTC Antibody in Western Blot (WB)

OTC Antibody in Western Blot (WB)

OTC Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Product Image Gallery

OTC Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using OTC (Product # PA5-28197) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

OTC Antibody in Western Blot (WB) — Western Blot using OTC Polyclonal Antibody (Product # PA5-28197). Sample (20 µg of whole cell lysate). Lane A: mouse Liver. 10% SDS PAGE. OTC Polyclonal Antibody (Product # PA5-28197) diluted at 1:30,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

OTC Antibody in Western Blot (WB) — Western Blot using OTC Polyclonal Antibody (Product # PA5-28197). Sample (30 µg of whole cell lysate). A: A431. 12% SDS PAGE. OTC Polyclonal Antibody (Product # PA5-28197) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

OTC Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Published figure using OTC polyclonal antibody (Product # PA5-28197) in Immunocytochemistry and Immunohistochemistry

产品信息

PA5-28197

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:30,000

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免疫组化 (IHC)

-

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

-

免疫组化（冰冻） (IHC (F))

Assay-dependent

-

免疫细胞化学 (ICC/IF)

-

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产品规格

种属反应

Human, Mouse

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 80 and 354 of Human OTC

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.65 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2545673

产品详细信息

Recommended positive controls: A431, HepG2, mouse Liver.

Predicted reactivity: Mouse (94%), Rat (93%), Pig (94%), Chicken (80%), Bovine (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This nuclear gene encodes a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Targeted mRNA Therapy for Ornithine Transcarbamylase Deficiency.

Molecular therapy : the journal of the American Society of Gene Therapy

Prieve MG,Harvie P,Monahan SD,Roy D,Li AG,Blevins TL,Paschal AE,Waldheim M,Bell EC,Galperin A,Ella-Menye JR,Houston ME

Published figure using OTC polyclonal antibody (Product # PA5-28197) in Immunohistochemistry

Wed Mar 07 00:00:00 UTC 2018

Targeted mRNA Therapy for Ornithine Transcarbamylase Deficiency.

Molecular therapy : the journal of the American Society of Gene Therapy

Prieve MG,Harvie P,Monahan SD,Roy D,Li AG,Blevins TL,Paschal AE,Waldheim M,Bell EC,Galperin A,Ella-Menye JR,Houston ME

Published figure using OTC polyclonal antibody (Product # PA5-28197) in Immunohistochemistry

Wed Mar 07 00:00:00 UTC 2018

Ataxin-2 (Atxn2)-Knock-Out Mice Show Branched Chain Amino Acids and Fatty Acids Pathway Alterations.

Molecular & cellular proteomics : MCP

Meierhofer D,Halbach M,Şen NE,Gispert S,Auburger G

PA528197 was used in western blot to suggest that Ataxin-2 modulates nutrition and metabolism

Sun May 01 00:00:00 UTC 2016

生物信息学

蛋白别名: Ornithine carbamoyltransferase, mitochondrial; ornithine transcarbamylase; Ornithine transcarbamylase, mitochondrial; OTCase; sparse fur

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基因别名: AI265390; OCTD; OTC; Sf; spf

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UniProt ID: (Human) P00480, (Mouse) P11725

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Entrez Gene ID: (Human) 5009, (Mouse) 18416

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ornithine carbamoyltransferase activity phospholipid binding amino acid binding phosphate ion binding transferase activity carboxyl- or carbamoyltransferase activity transferase metabolite interconversion enzyme

urea cycle liver development ornithine catabolic process midgut development response to zinc ion citrulline biosynthetic process response to insulin arginine biosynthetic process via ornithine response to drug anion homeostasis protein homotrimerization response to biotin ammonia homeostasis cellular amino acid metabolic process arginine biosynthetic process ornithine metabolic process cellular amino acid biosynthetic process