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OTUD7B Antibody (PA5-106333) in ICC/IF
Immunofluorescent analysis of OTUD7B in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and OTUD7B polyclonal antibody (Product # PA5-106333) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
产品信息
PA5-106333
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human OTUD7B(Accession Q6GQQ9), corresponding to amino acid residues D757-C807.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2854010
产品详细信息
Antibody detects endogenous levels of total OTUD7B.
靶标信息
Negative regulator of the non-canonical NF-kappa-B pathway that acts by mediating deubiquitination of TRAF3, an inhibitor of the NF-kappa-B pathway, thereby acting as a negative regulator of B-cell responses. In response to non-canonical NF-kappa-B stimuli, deubiquitinates 'Lys-48'-linked polyubiquitin chains of TRAF3, preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappa-B. Negatively regulates mucosal immunity against infections. Deubiquitinates ZAP70, and thereby regulates T cell receptor (TCR) signaling that leads to the activation of NF-kappa-B (PubMed:26903241). Plays a role in T cell homeostasis and is required for normal T cell responses, including production of IFNG and IL2. Mediates deubiquitination of EGFR (PubMed:22179831). Has deubiquitinating activity toward 'Lys-11', 'Lys-48' and 'Lys-63'-linked polyubiquitin chains (PubMed:27732584). Has a much higher catalytic rate with 'Lys-11'-linked polyubiquitin chains (in vitro); however the physiological significance of these data are unsure (PubMed:27732584). Hydrolyzes both linear and branched forms of polyubiquitin. [UniProt]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Cellular zinc finger anti-NF-kappa-B protein; cellular zinc finger anti-NF-kappaB Cezanne; Cezanne; OTU domain containing 7B; OTU domain-containing protein 7B; TRAF binding domain containing 2; Zinc finger A20 domain-containing protein 1; Zinc finger protein Cezanne; zinc finger, A20 domain containing 1
基因别名: 2900060B22Rik; 4930463P07Rik; AI462125; CEZANNE; OTUD7B; RGD1308111; Trabid2; ZA20D1
UniProt ID: (Human) Q6GQQ9, (Mouse) B2RUR8
Entrez Gene ID: (Human) 56957, (Rat) 310677, (Mouse) 229603
negative regulation of transcription from RNA polymerase II promoter
mucosal immune response
immune response
negative regulation of interleukin-8 production
protein K11-linked deubiquitination
negative regulation of I-kappaB kinase/NF-kappaB signaling
protein K63-linked deubiquitination
protein K48-linked deubiquitination
protein deubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of protein localization to nucleus
proteolysis
protein deubiquitination
immune system process
