Invitrogen

Occludin Polyclonal Antibody

Occludin Antibody in Immunocytochemistry (ICC/IF)

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Occludin Antibody (40-6100) in ICC/IF

Immunofluorescent analysis of occludin Antibody was done on 90% confluent log phase CACO2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Occludin Antibody (Product # 40-6100) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 45 minutes at room... View More

Occludin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Occludin Antibody in Immunoprecipitation (IP)

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产品信息

40-6100

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:10-1:100

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide derived from the N-terminal region of the human, rat, mouse, dog, and orangutan occludin proteins

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533473

靶标信息

Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Bacterial meningitis in the early postnatal mouse studied at single-cell resolution.

eLife

Wang J,Rattner A,Nathans J

40-6100 was used in Immunohistochemistry to imply that EC responses to infection are largely driven by the intrinsic EC response to LPS.

Thu Jun 15 00:00:00 UTC 2023

Occludin is a target of Src kinase and promotes lipid secretion by binding to BTN1a1 and XOR.

PLoS biology

Lu Y,Zhou T,Xu C,Wang R,Feng D,Li J,Wang X,Kong Y,Hu G,Kong X,Lu P

40-6100 was used in Immunoprecipitation, Immunohistochemistry (Frozen) to demonstrate that Ocln is a downstream target of Src kinase and promotes LD secretion by binding to BTN1a1 and XOR.

Sat Jan 01 00:00:00 UTC 2022

Occludin is a target of Src kinase and promotes lipid secretion by binding to BTN1a1 and XOR.

PLoS biology

Lu Y,Zhou T,Xu C,Wang R,Feng D,Li J,Wang X,Kong Y,Hu G,Kong X,Lu P

40-6100 was used in Immunoprecipitation, Immunohistochemistry (Frozen) to demonstrate that Ocln is a downstream target of Src kinase and promotes LD secretion by binding to BTN1a1 and XOR.

Sat Jan 01 00:00:00 UTC 2022

High-Dose Acetaminophen Alters the Integrity of the Blood-Brain Barrier and Leads to Increased CNS Uptake of Codeine in Rats.

Pharmaceutics

Yang J,Betterton RD,Williams EI,Stanton JA,Reddell ES,Ogbonnaya CE,Dorn E,Davis TP,Lochhead JJ,Ronaldson PT

40-6100 was used in Western Blot to show that BBB integrity is altered following a single high APAP dose, results that are relevant to patients abusing or misusing APAP and/or APAP/opioid combination products.

Wed Apr 27 00:00:00 UTC 2022

Effect of Bacillus subtilis Strains on Intestinal Barrier Function and Inflammatory Response.

Frontiers in immunology

Rhayat L,Maresca M,Nicoletti C,Perrier J,Brinch KS,Christian S,Devillard E,Eckhardt E

40-6100 was used in Western Blotting to assess the effect of three Bacillus-based probiotics on intestinal barrier function and intestinal inflammation.

Tue Aug 04 00:00:00 UTC 2020

Effect of Bacillus subtilis Strains on Intestinal Barrier Function and Inflammatory Response.

Frontiers in immunology

Rhayat L,Maresca M,Nicoletti C,Perrier J,Brinch KS,Christian S,Devillard E,Eckhardt E

40-6100 was used in Western Blotting to assess the effect of three Bacillus-based probiotics on intestinal barrier function and intestinal inflammation.

Tue Aug 04 00:00:00 UTC 2020

Beta-catenin signaling regulates barrier-specific gene expression in circumventricular organ and ocular vasculatures.

eLife

Wang Y,Sabbagh MF,Gu X,Rattner A,Williams J,Nathans J

40-6100 was used in Immunohistochemistry to study beta-catenin signalling in endothelial cells in high permeability vascular systems compared to barrier-competent endothelial cells.