Invitrogen

Occludin Polyclonal Antibody

Occludin Antibody in Immunohistochemistry (IHC)

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Occludin Antibody (PA5-20755) in IHC

Immunofluorescence of OCLN in human liver tissue with Occludin Polyclonal Antibody (Product # PA5-20755) at 20 µg/mL. Red: OCLN Blue: DAPI staining

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产品信息

PA5-20755

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

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免疫组化 (IHC)

2.5 µg/mL

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体外检测 (IV)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A 15 amino acid peptide from near the carboxy terminus of human OCLN.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_11154303

产品详细信息

A suggested positive control is human liver tissue lysate.

PA5-20755 can be used with blocking peptide PEP-0869.

靶标信息

Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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PA5-20755 was used in Western Blot to prove that LBP blocked the inhibitory effect on the proliferation activity of Sertoli cells after heat stress, reversed the dedifferentiation of Sertoli cells induced by heat stress, and ameliorated the structural integrity of the blood-testis barrier.