Invitrogen

Occludin Monoclonal Antibody (OC-3F10), HRP

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Occludin Antibody (33-1520) in WB

Knockout of Occludin was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR777079_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of Occludin was performed by loading 30 µg of Caco-2 Cas9 (Lane 1) andCaco-2 Occludin KO (Lane 2) membrane enriched extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlo... View More

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产品信息

33-1520

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

查看7篇已发表文章 7篇已发表文章

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

0.1-1.0 µg/mL

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Dog, Human, Mouse, Rat

已发表种属

Dog, Human, Mouse, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

OC-3F10

抗原

GST fusion protein consisting of the C-terminal region (~150aa) of human occludin.

查看免疫原

偶联物

HRP HRP HRP

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 4mg/mL BSA, 40% glycerol

内含物

0.19% Kathon™ CG

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533103

靶标信息

Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

HDAC inhibitors suppress the proliferation, migration and invasiveness of human head and neck squamous cell carcinoma cells via p63‑mediated tight junction molecules and p21‑mediated growth arrest.

Oncology reports

Kakiuchi A,Kakuki T,Ohwada K,Kurose M,Kondoh A,Obata K,Nomura K,Miyata R,Kaneko Y,Konno T,Kohno T,Himi T,Takano KI,Kojima T

33-1520 was used in Western Blotting to indicate that HDAC inhibitors suppress the proliferation, migration and invasiveness of HNSCC by downregulating the p63-mediated tight junction molecules JAM-A and claudin-1, and inducing p63 or p21-mediated growth arrest.

Thu Apr 01 00:00:00 UTC 2021

Astrocyte-derived VEGF increases cerebral microvascular permeability under high salt conditions.

Aging

Deng Z,Zhou L,Wang Y,Liao S,Huang Y,Shan Y,Tan S,Zeng Q,Peng L,Huang H,Lu Z

33-1520 was used in Western Blotting to reveal a new axis linking a high salt diet (HSD) to increased cerebral microvascular permeability through a VEGF-initiated inflammatory response.

Mon Jun 22 00:00:00 UTC 2020

Astrocyte-derived VEGF increases cerebral microvascular permeability under high salt conditions.

Aging

Deng Z,Zhou L,Wang Y,Liao S,Huang Y,Shan Y,Tan S,Zeng Q,Peng L,Huang H,Lu Z

33-1520 was used in Western Blotting to reveal a new axis linking a high salt diet (HSD) to increased cerebral microvascular permeability through a VEGF-initiated inflammatory response.

Mon Jun 22 00:00:00 UTC 2020

ApoE (Apolipoprotein E) in Brain Pericytes Regulates Endothelial Function in an Isoform-Dependent Manner by Modulating Basement Membrane Components.

Arteriosclerosis, thrombosis, and vascular biology

Yamazaki Y,Shinohara M,Yamazaki A,Ren Y,Asmann YW,Kanekiyo T,Bu G

33-1520 was used in Enzyme-linked immunosorbent assay to show that brain pericytes, the mural cells of the capillary walls, differentially modulate endothelial cell phenotype in an apoE isoform-dependent manner.

Wed Jan 01 00:00:00 UTC 2020

Fingolimod reduces circulating tight-junction protein levels and in vitro peripheral blood mononuclear cells migration in multiple sclerosis patients.

Scientific reports

Annunziata P,Cioni C,Masi G,Tassi M,Marotta G,Severi S

33-1520 was used in an ELISA assay to study the effects of fingolimod on circulating tight-junction protein levels as well as on peripheral blood mononuclear cells migration.

Thu Oct 18 00:00:00 UTC 2018

TNFα alters occludin and cerebral endothelial permeability: Role of p38MAPK.

PloS one

Ni Y,Teng T,Li R,Simonyi A,Sun GY,Lee JC

331520 was used in western blot to explore the responses of occludin to stimulation by TNFalpha, IL-1beta and LPS in an immortalized human cerebral endothelial cell line