Invitrogen

Occludin Recombinant Mouse Monoclonal Antibody (OC-3F10), Alexa Fluor™ Plus 488

Occludin Antibody in Immunocytochemistry (ICC/IF)

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Occludin Antibody (740006MP488) in ICC/IF

CRISPR-Cas9 mediated genome editing of Occludin (as confirmed by next generation sequencing) was achieved by using LentiArray™ Lentiviral sgRNA (Product # A32042, AssayID CRISPR777079_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Immunofluorescence analysis was performed on wild type Caco-2 cells (panel a-d), Caco-2 Cas9 control cells (panel e-h) and Caco-2 Cas9 cell line transduced with Occludin Lentiviral sgRNA cells (panel i-l). Cells were fixed, permeabilized, and labelled with Occludin Recombinant Mouse Monoclonal Antibody (O... View More

Occludin Antibody in Flow Cytometry (Flow)

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产品信息

740006MP488

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

1:2,000

流式细胞分析 (Flow)

0.063-0.25 µg/test

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

OC-3F10

抗原

GST fusion protein consisting of the C-terminal region (~150aa) of human occludin.

查看免疫原

偶联物

Alexa Fluor™ Plus 488 Alexa Fluor™ Plus 488 Alexa Fluor™ Plus 488

激发/发射光谱

493/518 nm 查看光谱

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Protein A/G

保存液

proprietary buffer, pH 6.5

内含物

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Wet ice

AB_3093154

产品详细信息

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

Applications Tested: This OC-3F10 antibody has been tested by immunocytochemistry and flow cytometric analysis of Caco-2 cells. This may be used for immunocytochemistry at 0.5 µg/mL and for flow cytometry at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 494 nm; Emission: 519 nm; Laser: Blue Laser

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.

仅用于科研。不用于诊断过程。未经明确授权不得转售。