Invitrogen

Osteocalcin Monoclonal Antibody (OC4-30)

Osteocalcin Antibody in Western Blot (WB)

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Osteocalcin Antibody (33-5400) in WB

International journal of molecular medicine 2020 - Figure 8 Endogenous protein expression of Runx2, COL1 and OCN during osteogenic differentiation of cPDLSCs. (A) Western blot analysis of protein expression levels of Runx2, COL1 and OCN in Vector-cPDLSCs and EfnB2-cPDLSCs after 7, 14 and 21 days of osteogenic/odontogenic induction. Quantification bands of (B) Runx2, (C) COL1 and (D) OCN in Vector-cPDLSCs and EfnB2-cPDLSCs. * P<0.05 vs. Vector-cPDLSCs. COL1,... View More

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产品信息

33-5400

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

10 µg/mL

免疫组化（冰冻） (IHC (F))

10 µg/mL

免疫细胞化学 (ICC/IF)

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产品规格

种属反应

Bovine, Dog, Chicken, Goat, Human, Sheep, Pig, Rabbit, Rat

已发表种属

Dog, Human, Rat

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

OC4-30

抗原

Full-length Bovine osteocalcin

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

2.0 mg/mL

纯化类型

Ion-exchange chromatography

保存液

PBS, pH 7.4, with 1% BSA

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533125

产品详细信息

Monoclonal antibody was obtained by fusing the mouse myeloma cell-line P3U1 with spleen cells of BALB/c mouse after immunization with bovine osteocalcin.

Specificity and Subclass: Epitope gamma-carboxylated residue at position 17 (does not react with decarboxylated osteocalcin.)

Reconstitution: Dissolve the lyophilized antibody in 50 µL of distilled water (final concentration: 2.0 mg/mL). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 µL or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.

Storage: 4°C This product does not contain preservative. The stock solution (2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles.

靶标信息

Osteocalcin belongs to the osteocalcin/matrix Gla-protein family and constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Osteocyte-Like Cells Differentiated From Primary Osteoblasts in an Artificial Human Bone Tissue Model.

JBMR plus

Munir A,Reseland JE,Tiainen H,Haugen HJ,Sikorski P,Christiansen EF,Reinholt FP,Syversen U,Solberg LB

33-5400 was used in Immunohistochemistry to establish cell culture conditions that favored the natural 3D orientation of the bone cells and stimulated the cascade of signaling needed for primary human osteoblasts to differentiate into osteocytes with the characteristically phenotypical dendritic network between cells.

Fri Sep 01 00:00:00 UTC 2023

Bidirectional ephrinB2‑EphB4 signaling regulates the osteogenic differentiation of canine periodontal ligament stem cells.

International journal of molecular medicine

Zhu S,Liu Z,Yuan C,Lin Y,Yang Y,Wang H,Zhang C,Wang P,Gu M

Published figure using Osteocalcin monoclonal antibody (Product # 33-5400) in Western Blot

Sun Mar 01 00:00:00 UTC 2020

Attachment and growth of dental pulp stem cells on dentin in presence of extra calcium.

Archives of oral biology

Özdal-Kurt F,Şen BH,Tuğlu I,Vatansever S,Türk BT,Deliloğlu-Gürhan I

33-5400 was used in immunocytochemistry to characterize the growth and attachment of dental pulp stem cells on dentin with extra calcium

Mon Aug 01 00:00:00 UTC 2016

The effect of different implant biomaterials on the behavior of canine bone marrow stromal cells during their differentiation into osteoblasts.

Biotechnic & histochemistry : official publication of the Biological Stain Commission

Özdal-Kurt F,Tuğlu I,Vatansever HS,Tong S,Şen BH,Deliloğlu-Gürhan SI

33-5400 was used in immunohistochemistry to elucidate the behavior of canine bone marrow stromal cells during differentiation into osteoblasts utilizing different implant biomaterials

Mon Aug 01 00:00:00 UTC 2016

The effect of autologous bone marrow stromal cells differentiated on scaffolds for canine tibial bone reconstruction.

Biotechnic & histochemistry : official publication of the Biological Stain Commission

Özdal-Kurt F,Tuğlu I,Vatansever HS,Tong S,Deliloğlu-Gürhan SI

33-5400 was used in immunohistochemistry to assess the use of cultured autologous bone marrow stromal cells on different scaffolds for healing defects in the tibias of dogs.

Thu Sep 01 00:00:00 UTC 2016

Effects of bisphosphonates on sutural bone formation and relapse: A histologic and immunohistochemical study.

American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics

Oztürk F,Babacan H,Inan S,Gümüş C

Fri Jul 01 00:00:00 UTC 2011

生物信息学

蛋白别名: BGP; bone gamma-carboxyglutamate (gla) protein; bone gamma-carboxyglutamate (gla) protein (osteocalcin); bone gamma-carboxyglutamate protein 2; bone gamma-carboxyglutamate protein osteocalcin; bone gamma-carboxyglutamate; BGLAP; bone gamma-carboxyglutamic acid (Gla) protein (osteocalcin); Bone Gla protein; bone gla protein BGP; bone Gla-protein; gamma-carbooxyglutamate (gla) protein; Gamma-carboxyglutamic acid-containing protein; Gla; Osteocalcin; PMF1; RP11-54H19.5; unnamed protein product

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基因别名: BGLAP; Bglap2; BGP; Bgpr; Bgpra; GLA; OC; OCN

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UniProt ID: (Human) P02818, (Bovine) P02820, (Rat) P04640