Invitrogen

Osteopontin Polyclonal Antibody

Osteopontin Antibody in Immunohistochemistry (IHC)

图: 1 / 7

Osteopontin Antibody (PA5-34579) in IHC

Immunohistochemistry of SPP1 in human bladder tissue with Osteopontin Polyclonal Antibody (Product # PA5-34579) at 5 µg/mL.

Osteopontin Antibody in Western Blot (WB)

Product Image Gallery

产品信息

PA5-34579

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

查看4篇已发表文章 4篇已发表文章

免疫组化 (IHC)

5 µg/mL

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

An 18 amino acid peptide near the amino terminus of human SPP1

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2551931

产品详细信息

A suggested positive control is human bladder tissue lysate.

PA5-34579 can be used with blocking peptide PEP-1622.

靶标信息

Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases close to the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and provide a novel substrate for plasmin and cathepsin D.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

SMOC2 promoted vascular smooth muscle cell proliferation, migration, and extracellular matrix degradation by activating BMP/TGF-β1 signaling pathway.

Journal of clinical biochemistry and nutrition

Wang X,Wang M,Zhou Z,Zou X,Song G,Zhang Q,Zhou H

PA5-34579 was used in Western Blotting to evaluate the function of SMOC2 in abdominal aortic aneurysm.

Fri Sep 01 00:00:00 UTC 2023

SMOC2 promoted vascular smooth muscle cell proliferation, migration, and extracellular matrix degradation by activating BMP/TGF-β1 signaling pathway.

Journal of clinical biochemistry and nutrition

Wang X,Wang M,Zhou Z,Zou X,Song G,Zhang Q,Zhou H

PA5-34579 was used in Western Blotting to evaluate the function of SMOC2 in abdominal aortic aneurysm.

Fri Sep 01 00:00:00 UTC 2023

MMP-3-mediated cleavage of OPN is involved in copper oxide nanoparticle-induced activation of fibroblasts.

Particle and fibre toxicology

Zhang Y,Mo Y,Zhang Y,Yuan J,Zhang Q

PA5-34579 was used in Western Blot to suggest that MMP-3-cleaved OPN may play a key role in Nano-CuO-induced activation of lung fibroblasts.

Mon May 22 00:00:00 UTC 2023

MMP-3-mediated cleavage of OPN is involved in copper oxide nanoparticle-induced activation of fibroblasts.

Particle and fibre toxicology

Zhang Y,Mo Y,Zhang Y,Yuan J,Zhang Q

PA5-34579 was used in Western Blot to suggest that MMP-3-cleaved OPN may play a key role in Nano-CuO-induced activation of lung fibroblasts.

Mon May 22 00:00:00 UTC 2023

Cell-directed aptamer therapeutic targeting for cancers including those within the central nervous system.

Oncoimmunology

Wei J,Song R,Sabbagh A,Marisetty A,Shukla N,Fang D,Najem H,Ott M,Long J,Zhai L,Lesniak MS,James CD,Platanias L,Curran M,Heimberger AB

Published figure using Osteopontin polyclonal antibody (Product # PA5-34579) in Western Blot

Tue Apr 19 00:00:00 UTC 2022

Comprehensive Proteomics Analysis of Stressed Human Islets Identifies GDF15 as a Target for Type 1 Diabetes Intervention.

Cell metabolism

Nakayasu ES,Syed F,Tersey SA,Gritsenko MA,Mitchell HD,Chan CY,Dirice E,Turatsinze JV,Cui Y,Kulkarni RN,Eizirik DL,Qian WJ,Webb-Robertson BM,Evans-Molina C,Mirmira RG,Metz TO

PA5-34579 was used in Immunohistochemistry to provides a unique resource for the identification of the human islet proteins regulated by cytokines and was effective in discovering a potential target for T1D therapy.

Tue Feb 04 00:00:00 UTC 2020

Comprehensive Proteomics Analysis of Stressed Human Islets Identifies GDF15 as a Target for Type 1 Diabetes Intervention.

Cell metabolism

Nakayasu ES,Syed F,Tersey SA,Gritsenko MA,Mitchell HD,Chan CY,Dirice E,Turatsinze JV,Cui Y,Kulkarni RN,Eizirik DL,Qian WJ,Webb-Robertson BM,Evans-Molina C,Mirmira RG,Metz TO

Tue Feb 04 00:00:00 UTC 2020

Comprehensive Proteomics Analysis of Stressed Human Islets Identifies GDF15 as a Target for Type 1 Diabetes Intervention.

Cell metabolism

Nakayasu ES,Syed F,Tersey SA,Gritsenko MA,Mitchell HD,Chan CY,Dirice E,Turatsinze JV,Cui Y,Kulkarni RN,Eizirik DL,Qian WJ,Webb-Robertson BM,Evans-Molina C,Mirmira RG,Metz TO

Tue Feb 04 00:00:00 UTC 2020

Comprehensive Proteomics Analysis of Stressed Human Islets Identifies GDF15 as a Target for Type 1 Diabetes Intervention.

Cell metabolism

Nakayasu ES,Syed F,Tersey SA,Gritsenko MA,Mitchell HD,Chan CY,Dirice E,Turatsinze JV,Cui Y,Kulkarni RN,Eizirik DL,Qian WJ,Webb-Robertson BM,Evans-Molina C,Mirmira RG,Metz TO

Tue Feb 04 00:00:00 UTC 2020

生物信息学

蛋白别名: 2AR; 44 kDa bone phosphoprotein; Bone sialoprotein 1; Bone sialoprotein 1 (BSPI/BSP1); Calcium oxalate crystal growth inhibitor protein; CALPHA1 fusion; Early T lymphocyte activation 1 (ETA1); early T-lymphocyte activation 1; Early T-lymphocyte activation 1 protein; HGNC:11255; immunoglobulin alpha 1 heavy chain constant region fusion protein; MGC110940; Minopontin; Nephropontin; Osteopontin; osteopontin-like protein; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; Secreted phosphoprotein 1; secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1); secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); Secreted phosphoprotein 1 (SPP1); secreted phosphoprotein 1 variant 6; Sialoprotein (osteopontin); SPP-1; SPP1/CALPHA1 fusion; urinar; Urinary stone protein; Uropontin

查看更多收起

基因别名: 2AR; 2b7; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1; OP; OPN; Opnl; OSP; PSEC0156; Ric; Spp-1; SPP1

查看更多收起

UniProt ID: (Human) P10451, (Mouse) P10923, (Rat) P08721

查看更多收起

Entrez Gene ID: (Human) 6696, (Mouse) 20750, (Rat) 25353

查看更多收起

功能

cytokine activity protein binding extracellular matrix binding cytokine

参与通路