NeoBiotechnologies

Osteopontin (OSP)/Secreted Phosphoprotein 1 (SPP1) Monoclonal Antibody (OSP/4589)

Osteopontin (OSP)/Secreted Phosphoprotein 1 (SPP1) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Osteopontin (OSP)/Secreted Phosphoprotein 1 (SPP1) Antibody (6696-RTM1-P1) in IHC (P)

IHC analysis of formalin-fixed, paraffin-embedded human prostate stained by Osteopontin Rat Monoclonal Antibody (OSP/4589) at 2 µg/mL in PBS. HIER: Tris/EDTA, pH9.0, 45 min. 2°: HRP-polymer, 30 min. DAB, 5 min.

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产品信息

6696-RTM1-P1

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

免疫组化（石蜡） (IHC (P))

1-2 µg/mL

免疫细胞化学 (ICC/IF)

1-2 µg/mL

流式细胞分析 (Flow)

1-2 µg/10^6 cells

产品规格

种属反应

Avian, Dog, Human, Mink, Mouse, Rabbit, Rat

宿主/亚型

Rat / IgG1

分类

Monoclonal

类型

Antibody

克隆号

OSP/4589

抗原

Recombinant full-length rat Spp1 protein

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

200 µg/mL

纯化类型

Protein A/G

保存液

PBS with 0.05% BSA

内含物

0.05% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody is stable for 24 months.

Positive Control: Bone. Found in plasma. Cellular Localization: Secreted.

Specificity Comments: Osteopontin is a bone matrix protein and a cytokine secreted by activated T cells. Osteopontin (OPN, also designated bone sialoprotein 1, urinary stone protein, spp-1, -1, nephropontin, uropontin) is an extracellular matrix cell adhesion phosphoglycoprotein. OPN is deposited into unmineralized matrix prior to calcification leading to localization at various tissue interfaces including cement lines, lamina limitans, and between collagen fibrils of fully matured hard tissues. While OPN is a major product of osteoblasts, it is also synthesized by brain and kidney cells. OPNs isolated from or secreted by various tissues have molecular weights due to post-translational modifications. OPN functions as a substrate for transglutaminase and is involved in cell adhesion, chemoattraction and immunomodulation.

靶标信息

Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases close to the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and provide a novel substrate for plasmin and cathepsin D.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 2AR; 44 kDa bone phosphoprotein; Bone sialoprotein 1; Bone sialoprotein 1 (BSPI/BSP1); Calcium oxalate crystal growth inhibitor protein; CALPHA1 fusion; Early T lymphocyte activation 1 (ETA1); early T-lymphocyte activation 1; Early T-lymphocyte activation 1 protein; HGNC:11255; immunoglobulin alpha 1 heavy chain constant region fusion protein; MGC110940; Minopontin; Nephropontin; OC-1; Osteopontin; osteopontin-like protein; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; Secreted phosphoprotein 1; secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1); secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); Secreted phosphoprotein 1 (SPP1); secreted phosphoprotein 1 variant 6; Sialoprotein (osteopontin); SPP-1; SPP1/CALPHA1 fusion; urinar; Urinary stone protein; Uropontin

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基因别名: 2AR; 2b7; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1; OP; OPN; Opnl; OSP; PSEC0156; Ric; Spp-1; SPP1