Invitrogen

P-cadherin Monoclonal Antibody (6A9)

P-cadherin Antibody in Immunocytochemistry (ICC/IF)

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P-cadherin Antibody (MA1-2003) in ICC/IF

Immunofluorescent analysis of placental Cadherin using Cadherin P Monoclonal Antibody (6A9) (Product # MA1-2003) shows staining in BEAS-2B Cells. Cadherin P (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Cadherin P (Produ... View More

P-cadherin Antibody in Western Blot (WB)

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产品信息

MA1-2003

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:1,000

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免疫组化（石蜡） (IHC (P))

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免疫细胞化学 (ICC/IF)

10 µg/mL

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免疫沉淀 (IP)

Assay-dependent

中和实验 (Neu)

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

6A9

抗原

A431 trypsinized membranes.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2077774

产品详细信息

MA1-2003 detects P-cadherin from human and mouse samples.

MA1-2003 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~120 kDa protein representing P-cadherin from A431 cell extract. MA1-2003 can also be used in immunoprecipitation and immunofluorescence procedures.

The MA1-2003 immunogen is A431 trypsinized membranes.

靶标信息

P-cadherin, also known as Cadherin-3, is a Type 1 cadherin protein that belongs to the cadherin superfamily. Type 1 cadherins are single-pass transmembrane proteins that have 5 extracellular cadherin repeats and an intracellular domain that binds p120-catenin and beta-catenin. Cadherins are calcium-dependent cell-cell adhesion glycoproteins responsible for a range of processes including development, wound healing, cell-cell signaling, cell growth and differentiation. P-cadherin is expressed in human fetal structures, and in adult tissues such as the basal layer of the epidermis, breast, prostate, ovary, cervix, hair follicle, and corneal endothelium. P-cadherin expression has also been reported on embryonic stem cells, and stem cells of the normal mammary gland, and hair follicle. Overexpression of P-cadherin has been associated with poor prognosis in breast, prostate, ovary, colon, and stomach carcinomas. Mutations in CDH3 are associated with congential hypotrichosis with juvenile macular dystrophy.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A Cdh3-β-catenin-laminin signaling axis in a subset of breast tumor leader cells control leader cell polarization and directional collective migration.

Developmental cell

Hwang PY,Mathur J,Cao Y,Almeida J,Ye J,Morikis V,Cornish D,Clarke M,Stewart SA,Pathak A,Longmore GD

MA1-2003 was used in Immunocytochemistry to highlight how a subset of leader cells interact with the microenvironment to direct collective migration.

Mon Jan 09 00:00:00 UTC 2023

Breast tumor IGF1R regulates cell adhesion and metastasis: alignment of mouse single cell and human breast cancer transcriptomics.

Frontiers in oncology

Obr AE,Bulatowicz JJ,Chang YJ,Ciliento V,Lemenze A,Maingrette K,Shang Q,Gallagher EJ,LeRoith D,Wood TL

MA1-2003 was used in Immunohistochemistry (Paraffin) to find that reduced IGF1R signaling in tumor epithelial cells dysregulates cadherin expression resulting in reduced cell adhesion.

Fri Feb 24 00:00:00 UTC 2023

Connexins, E-cadherin, Claudin-7 and β-catenin transiently form junctional nexuses during the post-natal mammary gland development.

Developmental biology

Dianati E,Poiraud J,Weber-Ouellette A,Plante I

MA1-2003 was used in western blot to assess the transiently formed junctional nexuses during post-natal mammary gland development by E-cadherins, connexins, beta-catenin, and claudin-7

Mon Aug 01 00:00:00 UTC 2016

Mechanism of extracellular domain-deleted dominant negative cadherins.

Journal of cell science

Nieman MT,Kim JB,Johnson KR,Wheelock MJ

MA1-2003 was used in western blot to investigate the functions of extracellular domain-deleted dominant-negative cadherin in cells

Sat May 01 00:00:00 UTC 1999

Inhibition of cadherin function differentially affects markers of terminal differentiation in cultured human keratinocytes.

Journal of cell science

Hines MD,Jin HC,Wheelock MJ,Jensen PJ

MA1-2003 was used in blocking/activating experiment to investigate the effect of inhibition of cadherin function on keratinocyte differentiation

Wed Dec 01 00:00:00 UTC 1999

生物信息学

蛋白别名: CADH3; cadherin 3, type 1, P-cadherin (placental); Cadherin-3; calcium-dependent adhesion protein, placental; P-cadherin; Placental cadherin; Placental-cadherin; RPE-specific cadherin

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基因别名: AI385538; Cadp; CDH3; CDHP; HJMD; P-cadherin; PCAD

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UniProt ID: (Human) P22223, (Mouse) P10287

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Entrez Gene ID: (Human) 1001, (Mouse) 12560

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功能

molecular_function calcium ion binding metal ion binding

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