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P38 Antibody (600-101-HB4) in IHC
Immunohistochemistry of Goat Anti-p38 Antibody. Tissue: Human Breast Cancer. Antigen Retrieval: HIER using Citrate Buffer for 20 min. Primary Antibody: Goat Anti-p38 at 1:100 for 30 min at RT. Secondary Antibody: Donkey Anti-Goat HRP Conjugated Antibody at 4µl/mL. Staining: DAB.
产品信息
600-101-HB4
产品规格
种属反应
Human,
Mouse
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Anti-p38 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human p38 conjugated to Keyhole Limpet Hemocyanin (KLH).
偶联物
形式
Liquid
浓度
0.95 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human p38. Blast analysis of this highly conserved protein shows 100% identity with mouse, chimpanzee, dog, and 92.3% with rat.
靶标信息
MAPK14 (p38 alpha) is a member of the stress-activated protein kinase class of MAPKs. The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CRK1; CSAID-binding protein; Csaids binding protein; cytokine suppressive anti-inflammatory drug binding protein; cytokine suppressive anti-inflammatory drug binding protein 1; Cytokine suppressive anti-inflammatory drug-binding protein; EC 2.7.11.24; ERK1; ERT2; MAP 14; MAP kinase 1; MAP kinase 14; MAP kinase MXI2; MAP kinase p38; MAP kinase p38 alpha; MAP kinase p38alpha; MAPK 14; MAPK1 antibody; MAX-interacting protein 2; mitogen activated protein kinase 14; Mitogen-activated protein kinase 14; Mitogen-activated protein kinase p38 alpha; MK14; OTTHUMP00000216563; OTTHUMP00000216566; p38 alpha; p38 MAP kinase; p38 MAP kinase alpha; p38 MAPK; p38 mitogen activated protein kinase; p38alpha Exip; RP1-179N16.5; SAPK2a; Stress-activated protein kinase 2a; tRNA synthetase cofactor p38
基因别名: Crk1; CSBP; CSBP1; CSBP2; CSPB1; EXIP; MAPK14; MXI2; p38; p38-alpha; p38a; p38ALPHA; p38MAPK; PRKM14; PRKM15; RK; SAPK2A
UniProt ID: (Human) Q16539, (Mouse) P47811
Entrez Gene ID: (Human) 1432, (Mouse) 26416
protein serine/threonine kinase activity
MAP kinase activity
MAP kinase kinase activity
protein binding
ATP binding
protein C-terminus binding
enzyme binding
protein phosphatase binding
NFAT protein binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
DNA damage checkpoint
activation of MAPK activity
cell morphogenesis
cartilage condensation
angiogenesis
placenta development
chondrocyte differentiation
glucose metabolic process
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
apoptotic process
movement of cell or subcellular component
chemotaxis
signal transduction
cell surface receptor signaling pathway
transmembrane receptor protein serine/threonine kinase signaling pathway
Ras protein signal transduction
skeletal muscle tissue development
response to glucose
positive regulation of gene expression
positive regulation of myotube differentiation
myoblast differentiation involved in skeletal muscle regeneration
peptidyl-serine phosphorylation
fatty acid oxidation
osteoclast differentiation
positive regulation of cyclase activity
lipopolysaccharide-mediated signaling pathway
response to muramyl dipeptide
intracellular signal transduction
cellular response to vascular endothelial growth factor stimulus
response to muscle stretch
p38MAPK cascade
positive regulation of protein import into nucleus
signal transduction in response to DNA damage
positive regulation of blood vessel endothelial cell migration
positive regulation of erythrocyte differentiation
positive regulation of myoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of glucose import
protein autophosphorylation
vascular endothelial growth factor receptor signaling pathway
regulation of sequence-specific DNA binding transcription factor activity
striated muscle cell differentiation
positive regulation of muscle cell differentiation
positive regulation of cardiac muscle cell proliferation
3'-UTR-mediated mRNA stabilization
cellular response to lipopolysaccharide
cellular response to ionizing radiation
negative regulation of canonical Wnt signaling pathway
positive regulation of brown fat cell differentiation
stress-induced premature senescence
cellular response to virus
regulation of cytokine production involved in inflammatory response
positive regulation of myoblast fusion
regulation of signal transduction by p53 class mediator
positive regulation of reactive oxygen species metabolic process
positive regulation of interleukin-12 secretion
regulation of transcription, DNA-templated
protein phosphorylation
cellular response to DNA damage stimulus
phosphorylation
response to lipopolysaccharide
stress-activated MAPK cascade
