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PABP Antibody (PA5-29883) in ICC/IF
Immunofluorescence analysis of PABP was performed using 70% confluent log phase HCT 116 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PABP Rabbit Polyclonal Antibody(Product # PA5-29883) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2703... View More
产品信息
PA5-29883
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 356 and 630 of Human PABP
偶联物
形式
Liquid
浓度
0.79 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2547357
产品详细信息
Recommended positive controls: 293T, A431, HeLa, HepG2, A549, H1299, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2.
Predicted reactivity: Mouse (99%), Rat (98%), Xenopus laevis (91%), Chicken (95%), Rhesus Monkey (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. In humans, the PABPs comprise a small nuclear isoform and a conserved gene family that displays at least 3 functional proteins: PABP1 (PABPC1), inducible PABP (iPABP, or PABPC4), and PABP3 (PABPC3). In addition, there are at least 4 pseudogenes, PABPCP1 to PABPCP4.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: PABP 1; PABP-1; poly A binding protein 1; poly(A) binding protein, cytoplasmic 1; poly(A) binding protein, cytoplasmic 2; poly(A)-binding protein 1; Polyadenylate-binding protein 1
基因别名: ePAB; PAB1; PABP; PABP1; PABPC1; PABPC2; PabpI; PABPL1
UniProt ID: (Human) P11940, (Rat) Q9EPH8, (Mouse) P29341
Entrez Gene ID: (Human) 26986, (Rat) 171350, (Mouse) 18458
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
mRNA processing
RNA splicing
gene silencing by RNA
positive regulation of nuclear-transcribed mRNA poly(A) tail shortening
positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
nuclear-transcribed mRNA poly(A) tail shortening
mRNA splicing, via spliceosome
mRNA polyadenylation
translational initiation
regulation of mRNA stability
positive regulation of viral genome replication
positive regulation of translation
mRNA stabilization
