Invitrogen

PABP Monoclonal Antibody (10E10)

PABP Antibody in Immunocytochemistry (ICC/IF)

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PABP Antibody (MA1-34079) in ICC/IF

Immunofluorescence analysis of PABP was performed using 70% confluent log phase HCT 116 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PABP Mouse Monoclonal Antibody(10E10) (Product # MA1-34079) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175)... View More

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PABP Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HCT 116 cells were transfected with PABP siRNA and reduction of signal was observed in Western Blot using PABP Monoclonal Antibody (Product # MA1-34079). {KD}

产品信息

MA1-34079

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

免疫细胞化学 (ICC/IF)

5 µg/mL

酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Chicken, Human, Rabbit, Xenopus

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

10E10

抗原

Recombinant PABP (Human) expressed from its 1.85 kbp cDNA, NcoI to SspI.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.1% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2156879

产品详细信息

MA1-34079 detects PABP from human, chicken, rabbit and Xenopus laevis samples. Does not react wtih Drosophilia melanogaster or mouse samples.

靶标信息

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. In humans, the PABPs comprise a small nuclear isoform and a conserved gene family that displays at least 3 functional proteins: PABP1 (PABPC1), inducible PABP (iPABP, or PABPC4), and PABP3 (PABPC3). In addition, there are at least 4 pseudogenes, PABPCP1 to PABPCP4.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: cytoplasmic poly(A)-binding protein 1-A; PABP-1; PABP-1-A; PABP-A; poly(A) binding protein, cytoplasmic 1; poly(A) binding protein, cytoplasmic 1 L homeolog; poly(A) binding protein, cytoplasmic 2; poly(A)-binding protein 1-A; Polyadenylate-binding protein 1; xPABP1-A

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基因别名: PAB1; PABP; PABP1; PABPC1; pabpc1-a; pabpc1-b; pabpc1.L; PABPC2; PABPL1; XELAEV_18033915mg

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UniProt ID: (Human) P11940