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Alomone Labs, LTD
PAR4 (F2RL3) (extracellular) Polyclonal Antibody
FIGURE: 1 / 6
PAR4 (F2RL3) (extracellular) Antibody (APR-034-200UL) in ICC/IF
Expression of PAR-4 in a human breast adenocarcinoma cell line - Cell surface detection of PAR-4 in a human breast adenocarcinoma cell line. A. Live intact human MCF-7 cells were stained with Anti-PAR4 (F2RL3) (extracellular) Antibody (#APR-034), (1:50), followed by goat Anti-rabbit-AlexaFluor-488 secondary Antibody (green). B. Live view of the cells. C. Nuclei were visualized with the cell-permeable dye Ho... View More
Product Details
APR-034-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)HLRGQRWPFGEAA(S)R, corresponding to amino acid residues 136-150 of human PAR-4, 1stextracellular loop
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
Coagulation factor II (thrombin) receptor-like 3 (F2RL3) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL3 is also a member of the protease-activated receptor family. F2RL3 is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. F2RL3 is activated by thrombin and trypsin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: coagulation factor II (thrombin) receptor-like 3; Coagulation factor II receptor-like 3; PAR-4; protease-activated receptor 4; protease-activated receptor-4; Proteinase-activated receptor 4; Thrombin receptor-like 3
Gene Aliases: F2RL3; PAR4
UniProt ID: (Human) Q96RI0, (Rat) Q920E0, (Mouse) O88634
Entrez Gene ID: (Human) 9002, (Rat) 116498, (Mouse) 14065
signal transduction
phospholipase C-activating G-protein coupled receptor signaling pathway
blood coagulation
response to wounding
platelet activation
positive regulation of release of sequestered calcium ion into cytosol
platelet dense granule organization
thrombin receptor signaling pathway
G-protein coupled receptor signaling pathway
hemostasis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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