Invitrogen

PARN Polyclonal Antibody

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PARN Antibody (PA5-30252) in WB

Western Blot using PARN Polyclonal Antibody (Product # PA5-30252). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. Lane B: BCL-1 . 7.5% SDS PAGE. PARN Polyclonal Antibody (Product # PA5-30252) diluted at 1:2,000.

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PARN Antibody — Silencing of PARN increased PLD2 protein expression. Cells were treated with transfection reagents only (Mock) or silenced with siRNA-negative control (Neg.) or with siRNA for PARN as indicated. Four days post-transfection, lysates were used for protein and gene expression analyses. (A-C) Protein expression for COS-7 cells and (F-H) for MCF-7 cells. Western blots are presented in A and F and the densitometry of PARN and PLD2 bands are shown in COS-7 cells (B-C) and MCF-7 cells (G-H). Actin was used as the equal protein loading control. (D,E) Gene expression for COS-7 cells and (I,J) for MCF-7 cells measured by RT-qPCR using the three housekeeping genes as indicated in the Fig. 1 legend. Data presented as bars are means+s.e.m. The difference between means was assessed by single-factor ANOVA. *P<0.05, significant increase between samples and controls; ##P<0.01, significant decrease between samples and controls. {KD}

产品信息

PA5-30252

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human, Mouse

已发表种属

Human, Non-human primate

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 256 of Human PARN

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2547726

产品详细信息

Recommended positive controls: NIH-3T3, BCL-1.

Predicted reactivity: Mouse (87%), Rat (86%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。