Invitrogen

PARP1 Polyclonal Antibody

PARP1 Antibody in Immunocytochemistry (ICC/IF)

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PARP1 Antibody (PA5-16452) in ICC/IF

Immunofluorescence analysis of PARP was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PARP (Poly ADP-Ribose Polymerase) Rabbit Polyclonal Antibody (Product # PA5-16452) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor®... View More

PARP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PARP1 Antibody in Immunohistochemistry (IHC)

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PARP1 Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Western blot analysis of PARP using PARP Polyclonal Antibody (Product # PA5-16452) shows increased expression of Cleaved PARP in HeLa and Jurkat cell line upon Staurosporine treatment. {TM}

产品信息

PA5-16452

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:20

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免疫细胞化学 (ICC/IF)

2 µg/mL

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide for the N-terminal region of human PARP

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_10978793

产品详细信息

PA5-16452 targets PARP (Poly ADP-Ribose Polymerase) in IHC (P) and WB applications and shows reactivity with mouse, Rat, and Human samples.

The PA5-16452 immunogen is a synthetic peptide for the N-terminal region of human PARP.

靶标信息

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Improved radiosynthesis of ¹²³I-MAPi, an auger theranostic agent.

International journal of radiation biology

Wilson TC,Jannetti SA,Guru N,Pillarsetty N,Reiner T,Pirovano G

PA5-16452 was used in Western Blotting to explore new method to synthesize 123I-MAPi in high activity yields (AY) and improved molar activities to facilitate clinical translation and multicenter trials.

Thu Jan 12 00:00:00 UTC 2023

[¹⁸F]PARPi Imaging Is Not Affected by HPV Status In Vitro.

Molecular imaging

Guru N,Demétrio De Souza França P,Pirovano G,Huang C,Patel SG,Reiner T

PA5-16452 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to explore whether HPV affects functional biomarkers, including γH2AX, RAD51, and PARP1.

Thu Oct 28 00:00:00 UTC 2021

Targeted Brain Tumor Radiotherapy Using an Auger Emitter.

Clinical cancer research : an official journal of the American Association for Cancer Research

Pirovano G,Jannetti SA,Carter LM,Sadique A,Kossatz S,Guru N,Demétrio De Souza França P,Maeda M,Zeglis BM,Lewis JS,Humm JL,Reiner T

PA5-16452 was used in Immunocytochemistry to explore potential of clinical translation of 123I-MAPi (Iodine-123 Meitner-Auger PARP1 inhibitor),which binds to the DNA repair enzyme PARP1, specifically targets cancer cells, sparing healthy tissue, and carries a radioactive payload within reach of the cancer cells' DNA.

Mon Jun 15 00:00:00 UTC 2020

Validation of the use of a fluorescent PARP1 inhibitor for the detection of oral, oropharyngeal and oesophageal epithelial cancers.

Nature biomedical engineering

Kossatz S,Pirovano G,Demétrio De Souza França P,Strome AL,Sunny SP,Zanoni DK,Mauguen A,Carney B,Brand C,Shah V,Ramanajinappa RD,Hedne N,Birur P,Sihag S,Ghossein RA,Gönen M,Strome M,Suresh A,Molena D,Ganly I,Kuriakose MA,Patel SG,Reiner T

PA5-16452 was used in Western Blot, Immunohistochemistry to report the validation, in animal models and a human, of the use of a previously described fluorescently labelled small-molecule inhibitor of the DNA repair enzyme poly(ADP-ribose) polymerase 1 (PARP1) for the detection of cancers of the oral cavity, pharynx and oesophagus.

Sun Mar 01 00:00:00 UTC 2020

Validation of the use of a fluorescent PARP1 inhibitor for the detection of oral, oropharyngeal and oesophageal epithelial cancers.

Nature biomedical engineering

Sun Mar 01 00:00:00 UTC 2020

Validation of the use of a fluorescent PARP1 inhibitor for the detection of oral, oropharyngeal and oesophageal epithelial cancers.

Nature biomedical engineering

Sun Mar 01 00:00:00 UTC 2020

Kaempferol Inhibits Zearalenone-Induced Oxidative Stress and Apoptosis via the PI3K/Akt-Mediated Nrf2 Signaling Pathway: In Vitro and In Vivo Studies.

International journal of molecular sciences

Rajendran P,Ammar RB,Al-Saeedi FJ,Mohamed ME,ElNaggar MA,Al-Ramadan SY,Bekhet GM,Soliman AM

PA5-16452 was used in Western Blotting to demonstrate that kaempferol possesses notable hepatoprotective roles against zearalenone-induced damage, potentially through the stimulation of Nrf2/HO-1 cascades and PI3K/Akt signalling.

Mon Dec 28 00:00:00 UTC 2020

Generation of Homogenous Three-Dimensional Pancreatic Cancer Cell Spheroids Using an Improved Hanging Drop Technique.

Tissue engineering. Part C, Methods

Ware MJ,Colbert K,Keshishian V,Ho J,Corr SJ,Curley SA,Godin B

PA5-16452 was used in immunohistochemistry - paraffin section to study the generation of homogenous three-dimensional pancreatic cancer cell spheroids and their improved hanging drop technique

Fri Apr 01 00:00:00 UTC 2016

生物信息学

蛋白别名: ADP-ribosyltransferase (NAD+; ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); ADPRT 1; ARTD1; DNA ADP-ribosyltransferase PARP1; msPARP; NAD(+) ADP-ribosyltransferase 1; NAD(+) pADPRT-1; PARP-1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly (ADP-ribose) polymerase); Poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) polymerase PARP-1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; poly[ADP-ribose] synthetase 1; Protein poly-ADP-ribosyltransferase PARP1

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基因别名: 5830444G22Rik; Adprp; ADPRT; ADPRT 1; ADPRT1; AI893648; ARTD1; C80510; pADPRT-1; PARP; PARP-1; PARP1; PPOL; sPARP-1

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UniProt ID: (Human) P09874, (Rat) P27008

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Entrez Gene ID: (Human) 142, (Rat) 25591, (Mouse) 11545

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